CDKN2AIP 抗体 (AA 183-689)
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北京 101111
Quick Overview for CDKN2AIP 抗体 (AA 183-689) (ABIN7871471)
抗原
See all CDKN2AIP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 183-689
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原理
- Carf Antibody / Collaborator of ARF / Cdkn2aip
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived mouse CDKN2AIP/CARF recombinant protein (Position: R183-T689) was used as the immunogen for the Carf antibody.
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亚型
- IgG
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anti-CDKN2A Interacting Protein (CDKN2AIP) (AA 1-579) antibody
CDKN2AIP 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (AA 1-579) antibodyCDKN2AIP 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1F7 unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (Internal Region) antibodyCDKN2AIP 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (AA 1-579) antibodyCDKN2AIP 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (AA 1-579) antibodyCDKN2AIP 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (AA 1-579) antibodyCDKN2AIP 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4D11 unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (C-Term) antibodyCDKN2AIP 适用: 人, 小鼠, 大鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (AA 470-519) antibodyCDKN2AIP 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, Pig, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (C-Term) antibodyCDKN2AIP 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CDKN2A Interacting Protein (CDKN2AIP) (N-Term) antibodyCDKN2AIP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Carf antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the Carf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- CDKN2AIP (CDKN2A Interacting Protein (CDKN2AIP))
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别名
- Carf
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背景
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Carf antibody detects Collaborator of ARF, a nuclear protein encoded by the CDKN2AIP gene on chromosome 4q35.1. CARF (CDKN2A-interacting protein) acts as a transcriptional co-regulator and cell cycle checkpoint factor that mediates the tumor suppressor function of p14ARF and p53. It plays key roles in cellular senescence, DNA damage response, and cell proliferation control. CARF is expressed in most proliferative tissues, including liver, kidney, and hematopoietic cells, with particularly high levels in dividing and stress-activated cells.
Structurally, CARF contains a coiled-coil domain and nuclear localization signals that facilitate interaction with chromatin-associated proteins and transcriptional regulators. It belongs to the CDKN2A-interacting protein family and acts as a bridge between the ARF tumor suppressor pathway and p53 signaling. CARF directly binds p14ARF and modulates p53 stability through MDM2 regulation, coordinating cell cycle arrest in response to genotoxic stress. Co-localization studies show CARF within the nucleus and nucleolus, where it associates with replication machinery and chromatin-modifying complexes.
Functionally, CARF serves as a cell cycle regulator that maintains p53 activity and prevents uncontrolled proliferation. Under DNA damage conditions, CARF enhances p53-dependent transcription of target genes such as CDKN1A (p21), promoting cell cycle arrest and senescence. It also regulates replication stress responses by interacting with checkpoint proteins such as ATR and CHK1. CARF expression levels determine whether a cell undergoes reversible arrest or permanent senescence, integrating multiple stress and growth signals.
Dysregulation of CARF expression has been implicated in cancer and aging. Overexpression can suppress proliferation and induce senescence, while loss of CARF may impair p53 function and enhance oncogenic transformation. In certain tumors, CARF mislocalization correlates with altered cell cycle control. Pathway associations include p53 signaling, DNA damage response, and cell cycle checkpoint regulation. Developmentally, CARF contributes to tissue homeostasis by regulating stem and progenitor cell proliferation.
The Carf antibody from NSJ Bioreagents is a valuable reagent for studies of tumor suppression, senescence, and p53 pathway regulation. -
UniProt
- Q8VHI4
抗原
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