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MYOD1 抗体 (AA 180-189)

The 小鼠 单克隆 anti-MYOD1 antibody (Clone SPM427) (ABIN7871437) specifically detects MYOD1 in IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7871437
发货至: 中国
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Quick Overview for MYOD1 抗体 (AA 180-189) (ABIN7871437)

抗原

See all MYOD1 抗体
MYOD1 (Myogenic Differentiation 1 (MYOD1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MYOD1 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM427
  • 抗原表位

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    AA 180-189

    原理

    Anti-MyoD1 Antibody

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.

    亚型

    IgG1, kappa
  • 应用备注

    The optimal dilution of the anti-MyoD1 antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the anti-MyoD1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    MYOD1 (Myogenic Differentiation 1 (MYOD1))

    别名

    MyoD1

    背景

    Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.

    UniProt

    P15172

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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