PIPOX 抗体 (AA 173-390)
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Quick Overview for PIPOX 抗体 (AA 173-390) (ABIN7871271)
抗原
See all PIPOX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 173-390
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原理
- PIPOX Antibody / Peroxisomal sarcosine oxidase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PIPOX recombinant protein (Position: E173-L390) was used as the immunogen for the PIPOX antibody.
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亚型
- IgG
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anti-Pipecolic Acid Oxidase (PIPOX) (AA 292-384) antibody
PIPOX 适用: 人 ELISA, WB, IP, IF 宿主: 小鼠 Monoclonal 3D1 unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) (AA 1-390) antibodyPIPOX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) (AA 259-337) antibodyPIPOX 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) antibodyPIPOX 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) (Internal Region) antibodyPIPOX 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) (AA 1-390) antibodyPIPOX 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) (AA 301-350) antibodyPIPOX 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 斑马鱼, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Pipecolic Acid Oxidase (PIPOX) (C-Term) antibodyPIPOX 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PIPOX antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PIPOX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PIPOX (Pipecolic Acid Oxidase (PIPOX))
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别名
- PIPOX
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背景
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PIPOX antibody detects Peroxisomal sarcosine oxidase, a flavin-dependent enzyme involved in the oxidative demethylation of sarcosine and pipecolate in peroxisomes. The UniProt recommended name is Peroxisomal sarcosine oxidase (PIPOX). This enzyme participates in amino acid catabolism and contributes to cellular redox homeostasis through hydrogen peroxide production during substrate oxidation.
Functionally, PIPOX antibody identifies a 438-amino-acid peroxisomal matrix enzyme that catalyzes the conversion of sarcosine to glycine, formaldehyde, and hydrogen peroxide. PIPOX contains a covalently bound FAD cofactor that mediates electron transfer to molecular oxygen during oxidative demethylation. It also oxidizes L-pipecolate, a lysine degradation intermediate, linking it to amino acid metabolism and detoxification of secondary amines.
The PIPOX gene is located on chromosome 17p13.3 and encodes a protein expressed in liver, kidney, and muscle tissues, where peroxisomal metabolism is active. PIPOX contributes to maintaining amino acid balance and redox stability by coupling substrate oxidation with reactive oxygen species generation. In peroxisomes, PIPOX works alongside catalase to neutralize hydrogen peroxide, preventing oxidative stress accumulation.
Physiologically, PIPOX supports one-carbon metabolism by producing glycine, a precursor for glutathione synthesis and methylation reactions. Its regulation is influenced by dietary amino acid intake, metabolic state, and oxidative stress levels. Reduced PIPOX activity disrupts amino acid degradation and increases susceptibility to oxidative injury. Elevated activity has been observed in metabolic adaptations to fasting and in response to peroxisome proliferator-activated receptor (PPAR) signaling.
PIPOX antibody is widely used in peroxisomal biology, amino acid metabolism, and redox regulation research. It is suitable for western blotting, immunohistochemistry, and enzyme localization assays to study PIPOX expression and function. This antibody supports studies of peroxisomal oxidative pathways, redox signaling, and metabolic adaptation. In biomedical research, PIPOX serves as a marker of peroxisomal enzymatic activity and amino acid turnover.
Structurally, PIPOX contains FAD-binding and substrate-binding domains typical of the flavin oxidase family. It localizes to peroxisomes via a C-terminal peroxisomal targeting signal (PTS1). NSJ Bioreagents provides PIPOX antibody reagents validated for use in amino acid metabolism, oxidative stress, and peroxisomal function research. -
UniProt
- Q9P0Z9
抗原
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