CD300a 抗体 (AA 156-299)
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Quick Overview for CD300a 抗体 (AA 156-299) (ABIN7871018)
抗原
See all CD300a (CD300A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 156-299
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原理
- CD300A Antibody
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human CD300A recombinant protein (Position: L156-T299) was used as the immunogen for the CD300A antibody.
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亚型
- IgG
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anti-CD300a (CD300A) (AA 18-180) antibody
CD300A 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8E4 unconjugated
anti-CD300a (CD300A) (AA 18-180) antibodyCD300A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD300a (CD300A) antibody (PE)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal P192 PE
anti-CD300a (CD300A) antibodyCD300A 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal MEM-260 unconjugated
anti-CD300a (CD300A) (AA 1-299) antibodyCD300A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD300a (CD300A) (Internal Region) antibodyCD300A 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD300a (CD300A) antibody (APC)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-260 APC
anti-CD300a (CD300A) antibody (PE)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-260 PE
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应用备注
- Optimal dilution of the CD300A antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the CD300A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- CD300a (CD300A)
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别名
- CD300A
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背景
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CD300A antibody detects Cluster of differentiation 300A, an inhibitory immunoreceptor expressed on myeloid and lymphoid cells that modulates immune activation and inflammation. Encoded by the CD300A gene on chromosome 17q25.1, this transmembrane glycoprotein belongs to the CD300 family of immunoglobulin-like receptors and plays a key role in maintaining immune homeostasis. CD300A contains a single extracellular Ig-like domain that recognizes phosphatidylserine and other lipids exposed on apoptotic cells, allowing immune cells to distinguish between healthy and dying cells.
Structurally, CD300A possesses multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic tail. Upon ligand engagement, these motifs recruit phosphatases such as SHP-1 and SHP-2, which dephosphorylate signaling intermediates and suppress proinflammatory responses. This mechanism dampens activation of monocytes, macrophages, mast cells, and NK cells, preventing excessive inflammation and autoimmune reactions. CD300A thereby acts as a negative regulator of immune activation by balancing activating signals from other CD300 family members.
The CD300A antibody is widely used in immunology, inflammation, and autoimmune disease research to study inhibitory receptor signaling, immune regulation, and apoptotic cell clearance. Flow cytometry and western blot analyses detect a 35-40 kilodalton band corresponding to CD300A, while immunohistochemistry shows strong membrane staining on leukocytes and myeloid cells. This antibody supports analysis of inhibitory receptor expression and function in both innate and adaptive immune systems.
Aberrant CD300A expression has been implicated in autoimmune diseases, allergy, and cancer. Increased expression can suppress antitumor immunity, while reduced expression contributes to hyperinflammatory conditions. The CD300A antibody provides a valuable tool for investigating the balance between immune activation and inhibition in health and disease. NSJ Bioreagents validates this antibody for its applications, ensuring reproducibility and sensitivity in immune signaling research. -
UniProt
- Q9UGN4
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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