DAK 抗体 (AA 15-538)
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Quick Overview for DAK 抗体 (AA 15-538) (ABIN7870797)
抗原
See all DAK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 15-538
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原理
- TKFC Antibody / Triokinase/FMN cyclase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human DAK/TKFC recombinant protein (Position: D15-E538) was used as the immunogen for the TKFC antibody.
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亚型
- IgG
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anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (AA 1-575) antibody
DAK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (Center) antibodyDAK 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (Internal Region) antibodyDAK 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (Internal Region) antibodyDAK 适用: 人, 小鼠, 大鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (AA 396-561) antibodyDAK 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (N-Term) antibodyDAK 适用: 人, 小鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (N-Term) antibodyDAK 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (AA 101-200) antibodyDAK 适用: 人 ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dihydroxyacetone Kinase 2 Homolog (DAK) (AA 1-575) antibodyDAK 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1D9 unconjugated
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应用备注
- Optimal dilution of the TKFC antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TKFC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- DAK (Dihydroxyacetone Kinase 2 Homolog (DAK))
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别名
- TKFC
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背景
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TKFC antibody detects Triokinase/FMN cyclase, a bifunctional enzyme involved in carbohydrate and flavin metabolism. TKFC catalyzes phosphorylation of glyceraldehyde and dihydroxyacetone to glyceraldehyde 3-phosphate and forms cyclic FMN from FMN, linking sugar and cofactor metabolism. The TKFC antibody is widely used in metabolic and enzymology research to study carbohydrate utilization, vitamin metabolism, and redox regulation.
TKFC is encoded by the TKFC gene located on human chromosome 17p13.1. The protein is approximately 585 amino acids long and localizes to the cytoplasm, particularly in liver and kidney tissues, where it supports energy metabolism and flavin cofactor recycling. TKFC functions as a bifunctional enzyme with separate kinase and cyclase domains, ensuring efficient coordination between sugar phosphorylation and FMN homeostasis.
The TKFC antibody detects a 63 kilodalton protein by western blot and shows cytoplasmic staining under immunofluorescence microscopy. TKFC participates in glycerol metabolism by converting trioses into intermediates of glycolysis and gluconeogenesis, thereby contributing to glucose homeostasis. Its FMN cyclase activity recycles flavin cofactors that support oxidative enzymes and mitochondrial redox balance.
Defects in TKFC activity lead to impaired energy metabolism, elevated triose intermediates, and disturbances in flavin homeostasis. Although rare, TKFC deficiency has been linked to metabolic acidosis and hepatic dysfunction. Elevated TKFC expression in tumors reflects metabolic adaptation to high glycolytic flux and enhanced redox cycling, supporting cell proliferation under hypoxia.
By connecting carbohydrate utilization and flavin metabolism, TKFC provides a unique window into cellular bioenergetics and redox control. NSJ Bioreagents provides a validated TKFC antibody optimized for western blot, immunofluorescence, and metabolic profiling, supporting research into energy metabolism, flavin biochemistry, and metabolic regulation. -
UniProt
- Q3LXA3
抗原
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