SMAP2 抗体 (AA 142-429)
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Quick Overview for SMAP2 抗体 (AA 142-429) (ABIN7870691)
抗原
See all SMAP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 142-429
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原理
- SMAP2 Antibody / Small ArfGAP 2
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SMAP2 recombinant protein (Position: K142-K429) was used as the immunogen for the SMAP2 antibody.
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亚型
- IgG
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anti-Small ArfGAP2 (SMAP2) (AA 111-140) antibody
SMAP2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB27373 unconjugated
anti-Small ArfGAP2 (SMAP2) (AA 379-429) antibodySMAP2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Small ArfGAP2 (SMAP2) (AA 227-276) antibodySMAP2 适用: 人, 大鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Small ArfGAP2 (SMAP2) (AA 110-140), (Middle Region) antibodySMAP2 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SMAP2 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SMAP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SMAP2 (Small ArfGAP2 (SMAP2))
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别名
- SMAP2
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背景
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SMAP2 antibody detects Small ArfGAP 2, an ADP-ribosylation factor GTPase-activating protein that regulates membrane trafficking and clathrin-mediated endocytosis. The UniProt recommended name is Small ArfGAP 2 (SMAP2). This cytosolic protein functions as a regulator of ARF family small GTPases, controlling vesicle formation and cargo sorting at the trans-Golgi network and plasma membrane.
Functionally, SMAP2 antibody identifies a 429-amino-acid protein containing an ArfGAP domain and a C-terminal clathrin-binding region. SMAP2 stimulates GTP hydrolysis on ARF1 and ARF6, facilitating vesicle uncoating and recycling. It plays a key role in synaptic vesicle turnover, receptor endocytosis, and intracellular trafficking, ensuring efficient communication between organelles.
The SMAP2 gene, located on chromosome 1p34.1, is widely expressed in brain, heart, and other tissues with active vesicular transport. In neurons, SMAP2 localizes to presynaptic terminals, where it interacts with clathrin and adaptor proteins to regulate synaptic vesicle cycling. Its activity coordinates endocytosis and exocytosis, maintaining synaptic plasticity and receptor availability.
Pathologically, aberrant SMAP2 expression or dysfunction may contribute to neurological disorders, including synaptic transmission defects and neurodevelopmental abnormalities. Its regulatory role in membrane dynamics also links it to cancer cell migration and secretion. Research with SMAP2 antibody provides insight into ARF signaling, vesicle recycling, and cellular communication mechanisms.
SMAP2 antibody is suitable for western blotting, immunocytochemistry, and immunoprecipitation to detect SMAP2 in cultured cells and tissues. NSJ Bioreagents offers validated SMAP2 antibody reagents designed for studies of ARF GTPase signaling, endocytosis, and neuronal function.
Structurally, SMAP2 contains an N-terminal ArfGAP catalytic domain that interacts with GTP-bound ARF, accelerating GTP hydrolysis, and a C-terminal region that binds clathrin and adaptor proteins. This modular structure allows SMAP2 to act as a molecular bridge between vesicle formation and signaling regulation. This antibody aids in understanding SMAP2i 1/2s contribution to intracellular trafficking and membrane remodeling. -
UniProt
- Q8WU79
抗原
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