MEIOB 抗体 (AA 140-442)
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Quick Overview for MEIOB 抗体 (AA 140-442) (ABIN7870665)
抗原
See all MEIOB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 140-442
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原理
- MEIOB Antibody / Meiosis-specific with OB domains protein
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human MEIOB recombinant protein (Position: I140-V442) was used as the immunogen for the MEIOB antibody.
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亚型
- IgG
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anti-Meiosis Specific with OB Domains (MEIOB) (Middle Region) antibody
MEIOB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Meiosis Specific with OB Domains (MEIOB) (C-Term) antibodyMEIOB 适用: 人, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Meiosis Specific with OB Domains (MEIOB) (Middle Region) antibodyMEIOB 适用: 人, 小鼠, 犬, 马, 兔, 大鼠, 豚鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Meiosis Specific with OB Domains (MEIOB) (C-Term) antibodyMEIOB 适用: 人, 小鼠, 犬, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MEIOB antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MEIOB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- MEIOB (Meiosis Specific with OB Domains (MEIOB))
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别名
- MEIOB
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背景
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MEIOB antibody detects Meiosis-specific with OB domains protein, a single-stranded DNA-binding protein essential for homologous recombination and meiotic crossover formation. The UniProt recommended name is Meiosis-specific with OB domains protein (MEIOB). This nuclear factor participates in the repair of programmed DNA double-strand breaks (DSBs) during meiosis, ensuring accurate chromosomal segregation and gamete genomic integrity.
Functionally, MEIOB antibody identifies a 471-amino-acid protein that localizes to meiotic chromatin in germ cells. MEIOB interacts with single-stranded DNA at resected DSBs and forms a complex with SPATA22, which stabilizes the DNA repair intermediate and promotes strand exchange facilitated by recombinases DMC1 and RAD51. Together, these interactions drive the homologous recombination events that generate genetic diversity during gametogenesis.
The MEIOB gene is located on chromosome 16p13.3 and is expressed exclusively in meiotic cells of the testis and ovary. MEIOB contains oligonucleotide/oligosaccharide-binding (OB) folds characteristic of single-stranded DNA-binding proteins. Its expression begins at the leptotene stage of meiosis and persists through pachytene, where it coordinates crossover formation and synapsis of homologous chromosomes. Loss of MEIOB function results in defective recombination, impaired synaptonemal complex formation, and meiotic arrest.
In mammals, MEIOB plays an indispensable role in meiotic DSB repair by stabilizing single-stranded DNA intermediates and promoting strand invasion. It also prevents excessive nuclease activity that could lead to genome instability. In humans, MEIOB mutations have been linked to infertility due to defective gametogenesis, emphasizing its essential contribution to reproductive success. In addition to its germline function, MEIOB expression may serve as a biomarker for spermatogenic activity and ovarian follicle development.
MEIOB antibody is widely used in reproductive biology, genetics, and DNA repair research. It is suitable for immunohistochemistry, immunoblotting, and chromatin association studies to track MEIOB localization and complex formation. This antibody supports investigations into meiotic recombination, chromosomal synapsis, and genetic stability. It is also used in infertility research to assess meiotic defects and germ cell differentiation.
Structurally, MEIOB possesses two OB-fold domains for DNA binding and forms a stoichiometric complex with SPATA22 that mimics the RPA-like architecture. NSJ Bioreagents provides MEIOB antibody reagents validated for use in meiosis, recombination, and fertility research. -
UniProt
- Q8N635
抗原
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