HCK 抗体 (AA 14-501)
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Quick Overview for HCK 抗体 (AA 14-501) (ABIN7870644)
抗原
See all HCK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 14-501
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原理
- HCK Antibody / Hematopoietic cell kinase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human HCK recombinant protein (Position: R14-R501) was used as the immunogen for the HCK antibody.
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亚型
- IgG
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anti-Hemopoietic Cell Kinase (HCK) (AA 2-526) antibody
HCK 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hemopoietic Cell Kinase (HCK) (AA 1-505) antibodyHCK 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Hemopoietic Cell Kinase (HCK) (AA 1-505) antibodyHCK 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Hemopoietic Cell Kinase (HCK) (AA 1-80) antibodyHCK 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2A6 unconjugated
anti-Hemopoietic Cell Kinase (HCK) antibodyHCK 适用: 人 WB, IF, IF (cc) 宿主: 小鼠 Monoclonal 8C1 unconjugated
anti-Hemopoietic Cell Kinase (HCK) (AA 1-505) antibodyHCK 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1D9-1A9 unconjugated
anti-Hemopoietic Cell Kinase (HCK) antibodyHCK 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 3D12E10 unconjugated
anti-Hemopoietic Cell Kinase (HCK) (Ser468) antibodyHCK 适用: 人, 小鼠, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hemopoietic Cell Kinase (HCK) (AA 1-220) antibodyHCK 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemopoietic Cell Kinase (HCK) (AA 1-220) antibodyHCK 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the HCK antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the HCK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- HCK (Hemopoietic Cell Kinase (HCK))
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别名
- HCK
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背景
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HCK antibody detects Hematopoietic cell kinase, a non-receptor tyrosine kinase encoded by the HCK gene on chromosome 20q11.21. HCK is a member of the Src family of kinases and is expressed predominantly in cells of the myeloid and B-lymphoid lineages, including macrophages, neutrophils, and monocytes. It plays essential roles in signal transduction pathways that regulate immune cell activation, adhesion, migration, and phagocytosis. HCK serves as a key mediator linking receptor engagement at the plasma membrane to downstream signaling cascades controlling inflammation and innate immunity.
Structurally, HCK contains an N-terminal myristoylation site for membrane attachment, followed by SH3 and SH2 domains involved in protein-protein interactions, and a C-terminal tyrosine kinase domain responsible for catalytic activity. It belongs to the Src kinase family, which includes LYN, FGR, and FYN, sharing conserved regulatory mechanisms. HCK exists in two isoforms (p59HCK and p61HCK) generated by alternative translation initiation, with p61HCK showing stronger membrane association and signaling potency.
Functionally, HCK transduces activation signals from immune receptors such as Fc gamma receptors, integrins, and toll-like receptors (TLRs). It phosphorylates downstream effectors including STAT3, PI3K, and MAPK pathway components, driving cytokine production and cell motility. HCK also interacts with cytoskeletal proteins to coordinate phagocytic cup formation and immune synapse assembly. Known substrates and partners include cortactin, paxillin, and focal adhesion kinase, supporting its role in cytoskeletal rearrangement during immune responses.
HCK participates in signaling pathways regulating inflammatory gene expression and antimicrobial defense. In macrophages, it mediates responses to lipopolysaccharide (LPS) through activation of NF-kappaB and p38 MAPK pathways. In B cells, HCK modulates B-cell receptor (BCR) signaling and survival. During development, HCK expression increases as myeloid progenitors differentiate into mature macrophages and neutrophils, highlighting its role in immune cell maturation.
Dysregulation of HCK activity is associated with immune disorders and cancer. Overexpression or constitutive activation has been observed in chronic myeloid leukemia and other hematologic malignancies, contributing to enhanced proliferation and resistance to apoptosis. Conversely, HCK deficiency impairs phagocytic and inflammatory responses. Pathway associations include JAK-STAT signaling, MAPK signaling, and Fc receptor-mediated phagocytosis. Pharmacological inhibition of HCK has shown potential in treating inflammatory and myeloproliferative diseases.
Immunohistochemical staining using HCK antibody shows cytoplasmic and membrane localization in macrophages, neutrophils, and B cells. The HCK antibody from NSJ Bioreagents is a reliable reagent for research on immune signaling, hematopoietic differentiation, and tyrosine kinase regulation. -
UniProt
- P08631
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途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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