Thymidine Phosphorylase 抗体 (AA 13-482)
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Quick Overview for Thymidine Phosphorylase 抗体 (AA 13-482) (ABIN7870479)
抗原
See all Thymidine Phosphorylase (TYMP) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-482
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原理
- TYMP Antibody / Thymidine phosphorylase / PD-ECGF
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PD-ECGF/TYMP recombinant protein (Position: P13-Q482) was used as the immunogen for the TYMP antibody.
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亚型
- IgG
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anti-Thymidine Phosphorylase (TYMP) (AA 311-482) antibody
TYMP 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-Thymidine Phosphorylase (TYMP) (AA 29-305) antibodyTYMP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thymidine Phosphorylase (TYMP) (AA 1-200) antibodyTYMP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymidine Phosphorylase (TYMP) (AA 1-482) antibodyTYMP 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Thymidine Phosphorylase (TYMP) antibodyTYMP 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal P-GF-44C unconjugated
anti-Thymidine Phosphorylase (TYMP) antibodyTYMP 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Monoclonal TYMP-2890R unconjugated Recombinant Antibody
anti-Thymidine Phosphorylase (TYMP) (N-Term) antibodyTYMP 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Thymidine Phosphorylase (TYMP) antibodyTYMP 适用: 人 WB, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Thymidine Phosphorylase (TYMP) (AA 13-254) antibodyTYMP 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Thymidine Phosphorylase (TYMP) (N-Term) antibodyTYMP 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TYMP antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TYMP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Thymidine Phosphorylase (TYMP)
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别名
- TYMP
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背景
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TYMP antibody detects Thymidine phosphorylase, also known as Platelet-derived endothelial cell growth factor (PD-ECGF), an enzyme encoded by the TYMP gene located on chromosome 22q13.33. TYMP is a cytoplasmic enzyme that catalyzes the reversible phosphorolysis of thymidine to thymine and 2-deoxy-D-ribose-1-phosphate, functioning in nucleotide metabolism and angiogenic signaling. It is widely expressed in endothelial cells, macrophages, and platelets, with particularly high levels observed in tumor vasculature and regenerative tissues. TYMP plays a dual role as a metabolic enzyme and a pro-angiogenic factor, linking DNA salvage pathways with vascular growth regulation.
As a key enzyme in pyrimidine salvage metabolism, TYMP maintains nucleotide pool balance for DNA synthesis and repair. Beyond its catalytic role, extracellular TYMP functions as PD-ECGF, promoting endothelial cell migration, angiogenesis, and wound healing. TYMP activity increases during hypoxia and tissue regeneration, facilitating the formation of new capillaries in response to injury or ischemia. Co-localization studies show TYMP associating with endothelial integrins and extracellular matrix components in angiogenic tissues.
Structurally, TYMP forms a homodimer with each subunit containing a thymidine-binding pocket and phosphate-binding residues essential for catalysis. It belongs to the thymidine phosphorylase family of pyrimidine salvage enzymes. The enzyme's catalytic activity also generates deoxyribose sugars that act as angiogenic mediators, linking metabolism to vascular signaling. TYMP interacts with molecules such as integrin alphavbeta3 and extracellular matrix proteins to enhance endothelial cell adhesion and migration.
Functionally, TYMP contributes to multiple biological processes, including angiogenesis, platelet activation, and oxidative stress response. Its expression is regulated by hypoxia-inducible factor 1-alpha (HIF-1alpha), cytokines, and growth factors such as VEGF and TNF-alpha. In cancer, TYMP expression is upregulated in tumor-associated macrophages and endothelial cells, promoting neovascularization and tumor progression. However, TYMP also exhibits cytotoxic effects in thymidine phosphorylase-deficient conditions by accumulating toxic thymidine metabolites, as seen in mitochondrial neurogastrointestinal encephalopathy (MNGIE).
Dysregulation of TYMP is clinically significant. Loss-of-function mutations cause MNGIE, a rare mitochondrial disorder characterized by gastrointestinal dysmotility, neuropathy, and leukoencephalopathy. Overexpression of TYMP is associated with poor prognosis in several cancers, including colorectal, breast, and gastric carcinoma, where it drives angiogenesis and tumor growth. Pathway involvement includes pyrimidine salvage metabolism, hypoxia response, and VEGF-mediated angiogenic signaling. In regenerative medicine, TYMP serves as a biomarker and target for promoting vascular repair and wound healing.
Immunohistochemical staining using TYMP antibody shows cytoplasmic and extracellular localization in endothelial and stromal cells. The TYMP antibody from NSJ Bioreagents is an excellent reagent for studies on nucleotide metabolism, angiogenesis, and tumor biology. -
UniProt
- P19971
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途径
- Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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