ERAS 抗体 (AA 13-42)
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Quick Overview for ERAS 抗体 (AA 13-42) (ABIN7870475)
抗原
See all ERAS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-42
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原理
- ERAS Antibody / GTPase ERas
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纯化方法
- Purified
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免疫原
- A portion of amino acids 13-42 from the human protein was used as the immunogen for the ERAS antibody.
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亚型
- Ig Fraction
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anti-ES cell expressed Ras (ERAS) antibody
ERAS 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 19-52), (N-Term) antibodyERAS 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB51538 unconjugated
anti-ES cell expressed Ras (ERAS) (AA 202-236), (C-Term) antibodyERAS 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB52380 unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (N-Term) antibodyERAS 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 13-42), (N-Term) antibodyERAS 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 1-233) antibodyERAS 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (N-Term) antibodyERAS 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 3-230) antibodyERAS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting points. Titration of the ERAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the ERAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- ERAS (ES cell expressed Ras (ERAS))
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别名
- ERAS
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背景
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
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UniProt
- Q7Z444
抗原
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