SIN3B 抗体 (AA 127-1127)
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北京 101111
Quick Overview for SIN3B 抗体 (AA 127-1127) (ABIN7870399)
抗原
See all SIN3B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 127-1127
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原理
- SIN3B Antibody / Paired amphipathic helix protein Sin3b
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SIN3B recombinant protein (Position: Q127-E1127) was used as the immunogen for the SIN3B antibody.
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亚型
- IgG
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anti-SIN3 Transcription Regulator Homolog B (SIN3B) (Internal Region) antibody
SIN3B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (AA 705-769) antibodySIN3B 适用: 人 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (Middle Region) antibodySIN3B 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Pig, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (AA 1063-1160) antibodySIN3B 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (AA 1-353) antibodySIN3B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (N-Term) antibodySIN3B 适用: 人, 小鼠, 大鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (AA 220-269) antibodySIN3B 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (AA 900-949) antibodySIN3B 适用: 人, 小鼠, Cow, 犬, 马, Pig, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (AA 614-663) antibodySIN3B 适用: 人, 小鼠, Cow, 犬, 马, Pig, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-SIN3 Transcription Regulator Homolog B (SIN3B) (Middle Region) antibodySIN3B 适用: 人, 小鼠, Cow, 斑马鱼, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SIN3B antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SIN3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))
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别名
- SIN3B
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背景
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SIN3B antibody detects Paired amphipathic helix protein Sin3b, a transcriptional corepressor that regulates chromatin structure and gene expression by recruiting histone deacetylase (HDAC) complexes. SIN3B functions in concert with its paralog SIN3A to coordinate gene silencing, differentiation, and cell cycle control. The SIN3B antibody is widely used in epigenetics and transcriptional regulation research to examine HDAC-dependent gene repression.
SIN3B is encoded by the SIN3B gene on human chromosome 19p13.11. The protein is approximately 150 kilodaltons and contains multiple paired amphipathic helix (PAH) domains that mediate interactions with transcription factors and chromatin-modifying enzymes. SIN3B serves as a scaffold that assembles HDAC1, HDAC2, RBBP4/7, and other corepressors into multiprotein complexes targeting specific promoters.
The SIN3B antibody detects a 150 kilodalton band by western blot and shows nuclear localization by immunofluorescence. Through recruitment of HDAC complexes, SIN3B deacetylates histones H3 and H4, resulting in chromatin compaction and transcriptional repression. It also interacts with transcription factors such as E2F4, MAD, and REST, enabling context-dependent regulation of genes involved in proliferation, senescence, and neuronal development.
SIN3B plays key roles in differentiation, acting as a switch from proliferation to quiescence. Knockdown of SIN3B leads to uncontrolled growth and defective chromatin silencing, while overexpression suppresses oncogenic transcription programs. Altered SIN3B function has been implicated in cancer, diabetes, and neurodevelopmental disorders due to disrupted epigenetic control.
As a master regulator of chromatin remodeling, SIN3B integrates multiple signaling pathways to maintain transcriptional balance. NSJ Bioreagents provides a validated SIN3B antibody optimized for western blot, chromatin immunohistochemistry, and nuclear complex analysis, supporting research into epigenetic repression, differentiation, and tumor suppression. -
UniProt
- O75182
抗原
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