PET112L 抗体 (AA 113-443)
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北京 101111
Quick Overview for PET112L 抗体 (AA 113-443) (ABIN7870152)
抗原
See all PET112L (PET112) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 113-443
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原理
- GATB Antibody / Glutamyl-tRNA amidotransferase subunit B
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human GATB recombinant protein (Position: R113-E443) was used as the immunogen for the GATB antibody.
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亚型
- IgG
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anti-PET112 Homolog (PET112) (AA 466-556) antibody
PET112 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B2 unconjugated
anti-PET112 Homolog (PET112) (AA 301-383) antibodyPET112 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-PET112 Homolog (PET112) (AA 101-200) antibodyPET112 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-PET112 Homolog (PET112) (AA 466-556) antibodyPET112 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-PET112 Homolog (PET112) (AA 34-61), (N-Term) antibodyPET112 适用: 人 WB 宿主: 兔 Polyclonal RB37741 unconjugated
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应用备注
- Optimal dilution of the GATB antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the GATB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PET112L (PET112) (PET112 Homolog (PET112))
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别名
- GATB
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背景
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GATB antibody detects Glutamyl-tRNA amidotransferase subunit B, a mitochondrial enzyme subunit essential for tRNA-dependent amino acid amidation. Encoded by the GATB gene on chromosome 4q31.3, this protein forms part of the heterotrimeric GatCAB complex, which converts misacylated Glu-tRNA(Gln) and Asp-tRNA(Asn) into correctly charged Gln-tRNA(Gln) and Asn-tRNA(Asn), respectively. This transamidation reaction ensures translational accuracy and maintains proper protein synthesis in mitochondria and prokaryote-like systems.
Structurally, GATB is a 471-amino-acid mitochondrial protein of approximately 52 kilodaltons that contains an amidase domain and ATP-binding sites required for the transamidation process. It associates with the catalytic subunit GATC and the tRNA recognition subunit GATA to form the functional GatCAB complex. Within mitochondria, GATB localizes to the matrix, where it couples amino acid activation with tRNA modification to sustain accurate translation of mitochondrial-encoded proteins.
The GATB antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, protein biosynthesis fidelity, and mitochondrial genome maintenance. Western blot analysis detects a 52 kilodalton band corresponding to GATB, while immunofluorescence shows punctate mitochondrial staining that colocalizes with mitochondrial matrix markers such as HSP60. This antibody provides a dependable reagent for monitoring translational control and enzyme complex integrity in mitochondria.
Functionally, GATB ensures translational fidelity by catalyzing the amination of misacylated tRNAs, preventing incorporation of incorrect amino acids during protein synthesis. Loss or mutation of GATB disrupts mitochondrial translation and compromises respiratory chain assembly, leading to mitochondrial dysfunction, energy deficiency, and developmental disorders. Altered expression of GATB has been linked to neurodegenerative conditions and metabolic syndromes associated with impaired mitochondrial translation. The GATB antibody enables mechanistic studies of mitochondrial gene expression, enzyme complex assembly, and translational regulation under physiological and stress conditions. NSJ Bioreagents validates this antibody for its applications, ensuring reliable detection in mitochondrial and metabolic research applications. -
UniProt
- O75879
抗原
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