TTLL5 抗体 (AA 111-1015)
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北京 101111
Quick Overview for TTLL5 抗体 (AA 111-1015) (ABIN7870111)
抗原
See all TTLL5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 111-1015
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原理
- TTLL5 Antibody / Tubulin tyrosine ligase-like protein 5
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human TTLL5 recombinant protein (Position: R111-E1015) was used as the immunogen for the TTLL5 antibody.
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亚型
- IgG
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anti-Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5) antibody
TTLL5 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5) (AA 1231-1281) antibodyTTLL5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5) (AA 111-1015) antibodyTTLL5 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TTLL5 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TTLL5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- TTLL5 (Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5))
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别名
- TTLL5
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背景
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TTLL5 antibody detects Tubulin polyglutamylase TTLL5 (Tubulin tyrosine ligase-like protein 5), an enzyme that catalyzes post-translational glutamylation of alpha- and beta-tubulin, thereby regulating microtubule stability, motility, and interactions with associated proteins. Encoded by the TTLL5 gene on chromosome 14q24.3, this enzyme belongs to the TTLL family of tubulin-modifying enzymes responsible for adding glutamate side chains to tubulin and other cytoskeletal proteins. TTLL5 contains a tubulin-tyrosine ligase domain and a coiled-coil region that mediate enzymatic activity and substrate recognition. Through microtubule glutamylation, TTLL5 plays crucial roles in ciliary function, intracellular trafficking, and sensory perception.
TTLL5 is expressed in multiple tissues, including retina, testis, and brain, where it supports specialized microtubule structures such as photoreceptor connecting cilia and sperm flagella. In photoreceptor cells, TTLL5 regulates the glutamylation of ciliary axonemes and outer segment proteins critical for vision. Mutations in TTLL5 cause inherited retinal dystrophies, including cone-rod dystrophy and Leber congenital amaurosis, due to defective ciliary transport and photoreceptor degeneration. In spermatozoa, TTLL5 is essential for flagellar stability and motility, and its absence leads to asthenozoospermia and male infertility.
The TTLL5 antibody is widely used in cell biology, neurobiology, and reproductive research to study microtubule post-translational modification and ciliary biology. Western blot analysis identifies a 130 kilodalton band corresponding to the full-length enzyme, while immunofluorescence reveals localization along ciliary and cytoplasmic microtubules. Researchers employ this antibody to investigate TTLL5-dependent pathways controlling axonemal function and intracellular transport. Dysregulation of tubulin glutamylation has been implicated in neurodegenerative diseases and ciliopathies, making TTLL5 an important molecular marker for cytoskeletal regulation.
Functionally, TTLL5 acts with other TTLL family members to maintain the dynamic balance between glutamylation and deglutamylation, which modulates motor protein activity and vesicular transport. The TTLL5 antibody facilitates the study of these processes and enables precise mapping of TTLL5 expression across tissues. NSJ Bioreagents supplies this antibody validated for its applications, ensuring reliable detection of TTLL5 in studies of ciliary structure, microtubule regulation, and human disease mechanisms. -
UniProt
- Q6EMB2
抗原
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