NCAPG 抗体 (AA 1-993)
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Quick Overview for NCAPG 抗体 (AA 1-993) (ABIN7869671)
抗原
See all NCAPG 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-993
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原理
- NCAPG Antibody / Non-SMC condensin I complex subunit G
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NCAPG recombinant protein (Position: M1-R993) was used as the immunogen for the NCAPG antibody.
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亚型
- IgG
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anti-Non-SMC Condensin I Complex, Subunit G (NCAPG) (AA 336-435) antibody
NCAPG 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4B1 unconjugated
anti-Non-SMC Condensin I Complex, Subunit G (NCAPG) (AA 161-170) antibodyNCAPG 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit G (NCAPG) (C-Term) antibodyNCAPG 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit G (NCAPG) (AA 117-145), (N-Term) antibodyNCAPG 适用: 人 WB 宿主: 兔 Polyclonal RB40395 unconjugated
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应用备注
- Optimal dilution of the NCAPG antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NCAPG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NCAPG (Non-SMC Condensin I Complex, Subunit G (NCAPG))
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别名
- NCAPG
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背景
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NCAPG antibody detects Non-SMC condensin I complex subunit G, an essential component of the condensin I complex that mediates chromosome condensation and segregation during mitosis. The UniProt recommended name is Non-SMC condensin I complex subunit G (NCAPG), with alternate names condensin complex subunit G, chromosome-associated protein G, and CAP-G. NCAPG functions as a regulatory subunit within the condensin complex, coordinating chromosome supercoiling and stabilization necessary for accurate chromosomal segregation.
During cell division, NCAPG antibody identifies a protein critical for organizing chromatin architecture. NCAPG, together with SMC2, SMC4, NCAPH, and NCAPD2, composes condensin I, which compacts chromatin fibers into mitotic chromosomes. It binds ATP-dependent SMC core units and interacts with regulatory partners that modulate DNA topology and cohesion release. In addition to its structural role, NCAPG has been linked to transcriptional regulation, genomic stability, and cell proliferation pathways. Abnormal expression of NCAPG correlates with aneuploidy, mitotic defects, and tumor progression in various cancers, including hepatocellular carcinoma, glioblastoma, and lung cancer.
The NCAPG antibody is used to monitor cell cycle progression, particularly in the G2/M phase where condensin activation occurs. Knockdown of NCAPG leads to chromosome segregation errors, DNA damage, and mitotic arrest. Studies also show NCAPG involvement in DNA repair and replication stress responses. The NCAPG gene is located on chromosome 4p15.32 and encodes a 1015-amino acid nuclear protein. NCAPG's expression peaks in proliferating cells and declines upon differentiation, making it a useful marker of mitotic activity.
Beyond its canonical mitotic role, NCAPG contributes to chromatin reorganization during transcriptional activation and may interact with epigenetic regulators. Upregulation of NCAPG in cancer promotes oncogenic growth via activation of PI3K/AKT and MAPK signaling pathways. High NCAPG levels are associated with poor prognosis and therapeutic resistance, suggesting it as a biomarker and target for anti-proliferative therapy. Researchers use NCAPG antibody for western blotting, immunostaining, and flow cytometry to study mitotic chromosome structure and cell cycle regulation. NSJ Bioreagents offers validated reagents for detecting human NCAPG to support research in cell division and chromosomal biology. -
UniProt
- Q9BPX3
抗原
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