TIMM9 抗体 (AA 1-89)
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北京 101111
Quick Overview for TIMM9 抗体 (AA 1-89) (ABIN7869606)
抗原
See all TIMM9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-89
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原理
- TIMM9 Antibody / Tim9
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human TIMM9 recombinant protein (Position: M1-R89) was used as the immunogen for the TIMM9 antibody.
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亚型
- IgG
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anti-Translocase of Inner Mitochondrial Membrane 9 (TIMM9) (AA 1-89) antibody
TIMM9 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 1D6 unconjugated
anti-Translocase of Inner Mitochondrial Membrane 9 (TIMM9) (C-Term) antibodyTIMM9 适用: 人, 大鼠, 小鼠 IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Translocase of Inner Mitochondrial Membrane 9 (TIMM9) (AA 2-89) antibodyTIMM9 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Translocase of Inner Mitochondrial Membrane 9 (TIMM9) (AA 18-44) antibodyTIMM9 适用: 人 WB 宿主: 兔 Polyclonal RB37475 unconjugated
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应用备注
- Optimal dilution of the TIMM9 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TIMM9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- TIMM9 (Translocase of Inner Mitochondrial Membrane 9 (TIMM9))
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别名
- TIMM9
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背景
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TIMM9 antibody detects Mitochondrial import inner membrane translocase subunit Tim9, a chaperone essential for importing hydrophobic carrier proteins into the mitochondrial inner membrane. Encoded by the TIMM9 gene on chromosome 14q24.3, this small protein is part of the TIM9-TIM10 complex that guides precursor proteins through the mitochondrial intermembrane space to their insertion sites. TIMM9 maintains protein folding stability during transit and prevents aggregation of hydrophobic substrates prior to membrane integration.
Structurally, TIMM9 is an 89-amino-acid protein of approximately 10 kilodaltons containing twin CX3C motifs that coordinate zinc ions to stabilize its characteristic hexameric structure. Within the intermembrane space, TIMM9 forms a heterohexameric complex with TIMM10 or TIMM10B, providing a soluble chaperone system that transfers imported precursors from the TOM complex (translocase of the outer membrane) to the TIM22 complex for membrane insertion.
The TIMM9 antibody is widely used in mitochondrial import, bioenergetics, and protein trafficking research to study mitochondrial assembly, carrier translocation, and protein folding. Western blot analysis detects a 10 kilodalton band corresponding to TIMM9, while immunofluorescence demonstrates mitochondrial localization colocalizing with markers such as TOM20 and COX IV. This antibody is valuable for analyzing mitochondrial protein import efficiency and intermembrane space chaperone dynamics.
Functionally, TIMM9 is essential for the import of metabolite carrier proteins, including members of the ADP/ATP translocase and phosphate carrier families. Loss of TIMM9 disrupts carrier protein biogenesis, resulting in impaired mitochondrial respiration and metabolic deficiency. Defects in TIMM9 expression or complex formation have been implicated in mitochondrial encephalopathies and neurodegenerative diseases characterized by energy failure and oxidative stress. The TIMM9 antibody provides a sensitive reagent for evaluating mitochondrial import machinery integrity and studying molecular chaperone systems that maintain organelle function. NSJ Bioreagents validates this antibody for its applications, ensuring specificity and reproducibility for mitochondrial protein import studies. -
UniProt
- Q9Y5J7
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途径
- Sensory Perception of Sound, SARS-CoV-2 Protein Interactome
抗原
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