N4BP1 抗体 (AA 1-885)
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北京 101111
Quick Overview for N4BP1 抗体 (AA 1-885) (ABIN7869602)
抗原
See all N4BP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-885
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原理
- N4BP1 Antibody / NEDD4-binding protein 1 / KIAA0615
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纯化方法
- Antigen affinity purified
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免疫原
- E. coli-derived recombinant human protein (amino acids M1-K885) was used as the immunogen for the N4BP1 antibody.
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亚型
- IgG
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anti-NEDD4 Binding Protein 1 (N4BP1) antibody
N4BP1 适用: 小鼠 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB120 unconjugated Recombinant Antibody
anti-NEDD4 Binding Protein 1 (N4BP1) (AA 190-590) antibodyN4BP1 适用: 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (Internal Region) antibodyN4BP1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (AA 1-896) antibodyN4BP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (AA 250-300) antibodyN4BP1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (AA 383-432) antibodyN4BP1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the N4BP1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the N4BP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- N4BP1 (NEDD4 Binding Protein 1 (N4BP1))
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别名
- N4BP1
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背景
- Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73?, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73? for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73? and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.
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UniProt
- O75113
抗原
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