RPS21 抗体 (AA 1-83)
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北京 101111
Quick Overview for RPS21 抗体 (AA 1-83) (ABIN7869573)
抗原
See all RPS21 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-83
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原理
- RPS21 Antibody / 40S ribosomal protein S21
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human RPS21 recombinant protein (Position: M1-F83) was used as the immunogen for the RPS21 antibody.
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亚型
- IgG
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anti-Ribosomal Protein S21 (RPS21) (AA 1-81), (Ser21) antibody
RPS21 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S21 (RPS21) (AA 44-71), (C-Term) antibodyRPS21 适用: 人 WB 宿主: 兔 Polyclonal RB43567 unconjugated
anti-Ribosomal Protein S21 (RPS21) (Internal Region) antibodyRPS21 适用: 人, 小鼠, 大鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S21 (RPS21) (C-Term) antibodyRPS21 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, Bat, Hamster, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S21 (RPS21) (N-Term) antibodyRPS21 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 斑马鱼, Bat, Hamster, Pig, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S21 (RPS21) (N-Term) antibodyRPS21 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S21 (RPS21) (AA 33-83) antibodyRPS21 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S21 (RPS21) (AA 1-50) antibodyRPS21 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the RPS21 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the RPS21 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- RPS21 (Ribosomal Protein S21 (RPS21))
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别名
- RPS21
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背景
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RPS21 antibody detects 40S ribosomal protein S21, encoded by the RPS21 gene on chromosome 20p11.23. RPS21 antibody is used in studies of ribosome assembly, translation, and protein synthesis regulation. RPS21 is a component of the small ribosomal subunit (40S) and contributes to decoding mRNA and ensuring fidelity of translation. Expression is ubiquitous across tissues, reflecting the essential requirement for ribosomal proteins in cell growth and proliferation. Mutations in ribosomal protein genes, including RPS21, are associated with ribosomopathies and cancer.
Structurally, RPS21 is a small basic protein that integrates into the head region of the 40S ribosomal subunit. It contributes to the architecture of the decoding center and interacts with ribosomal RNA and other ribosomal proteins. Structural studies place RPS21 near the mRNA entry channel, supporting its role in guiding and stabilizing mRNA during translation. It is encoded by a highly conserved gene, underscoring its fundamental role in ribosome function.
Functionally, RPS21 ensures proper initiation and elongation of protein synthesis. By contributing to ribosomal stability, it influences translation efficiency and fidelity. Knockdown of RPS21 reduces global protein synthesis and triggers nucleolar stress responses. Deficiency activates p53 pathways and can impair cell proliferation, linking RPS21 to cell cycle control. Researchers employ RPS21 antibody to study ribosome biogenesis, translational control, and stress responses.
Clinically, RPS21 mutations have been identified in Diamond-Blackfan anemia, a congenital ribosomopathy characterized by bone marrow failure and developmental abnormalities. Somatic alterations in RPS21 expression are also observed in cancers, particularly hematologic malignancies, where dysregulated protein synthesis supports tumor growth. Elevated expression correlates with poor prognosis in some tumors. NSJ Bioreagents provides RPS21 antibody to facilitate research into ribosome biology, disease mechanisms, and translational regulation.
Experimentally, RPS21 antibody is used in western blotting to detect the ~9 kDa protein, in immunohistochemistry to study nucleolar expression, and in immunofluorescence microscopy to localize it within ribosomes. Immunoprecipitation with RPS21 antibody enables analysis of ribosomal complexes and translation machinery. -
UniProt
- P63220
抗原
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