NLE1 抗体 (AA 1-385)
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Quick Overview for NLE1 抗体 (AA 1-385) (ABIN7869280)
抗原
See all NLE1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-385
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原理
- NLE1 Antibody / Notchless protein homolog 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NLE1 recombinant protein (Position: M1-R385) was used as the immunogen for the NLE1 antibody.
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亚型
- IgG
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anti-Notchless Homolog 1 (NLE1) (AA 206-485) antibody
NLE1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Notchless Homolog 1 (NLE1) (Internal Region) antibodyNLE1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Notchless Homolog 1 (NLE1) (AA 206-485) antibodyNLE1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Notchless Homolog 1 (NLE1) (AA 100-150) antibodyNLE1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Notchless Homolog 1 (NLE1) (C-Term) antibodyNLE1 适用: 人, 小鼠, 大鼠, 猴, 豚鼠, 马, Cow, 犬, 兔, 斑马鱼, Bat, 小鸡, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Notchless Homolog 1 (NLE1) (AA 351-400) antibodyNLE1 适用: 人, 小鼠, 大鼠, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Notchless Homolog 1 (NLE1) (AA 343-372), (C-Term) antibodyNLE1 适用: 人 WB 宿主: 兔 Polyclonal RB33320 unconjugated
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应用备注
- Optimal dilution of the NLE1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NLE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NLE1 (Notchless Homolog 1 (NLE1))
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别名
- NLE1
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背景
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The NLE1 antibody targets Notchless protein homolog 1, a nucleolar assembly factor encoded by the NLE1 gene. Notchless protein homolog 1 is required for ribosome biogenesis, pre-60S subunit maturation, and proper cell-cycle progression. Originally identified for its genetic interaction with the Notch pathway, NLE1 participates in both ribosomal RNA processing and developmental signaling. The NLE1 antibody enables researchers to study nucleolar organization, protein-synthesis control, and cross-talk between growth and differentiation pathways.
Notchless protein homolog 1 contains WD-repeat motifs that facilitate interactions with nucleolar proteins involved in ribosome assembly, including NOP7 and RPF2. It promotes 60S subunit maturation by assisting in rRNA folding and ribosomal protein incorporation. The NLE1 antibody supports localization studies showing strong nucleolar enrichment, consistent with its essential role in ribosome production. Depletion of NLE1 results in defective ribosome biogenesis, p53 activation, and cell-cycle arrest.
During embryonic development, Notchless protein homolog 1 influences cell-fate determination through modulation of Notch receptor signaling. Loss of NLE1 disrupts somite segmentation and neurogenesis in animal models. The NLE1 antibody allows analysis of these regulatory interactions, helping delineate how nucleolar activity intersects with developmental gene networks. Its dual role in ribosome formation and signaling regulation underscores the integration of biosynthetic and morphogenetic processes.
In cancer research, altered ribosome biogenesis and nucleolar stress contribute to tumorigenesis. Notchless protein homolog 1 expression correlates with proliferative capacity, making it a useful marker for studies of nucleolar dynamics in malignant tissues. The NLE1 antibody supports evaluation of nucleolar size, rRNA synthesis rate, and ribosomal assembly integrity in cancer and stress-response models.
The NLE1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing prominent nucleolar staining. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for ribosome biology, developmental genetics, and cancer research. By enabling detailed analysis of Notchless protein homolog 1, the NLE1 antibody advances understanding of ribosomal assembly pathways and their coordination with developmental and oncogenic signaling. -
UniProt
- Q9NVX2
抗原
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