NSUN4 抗体 (AA 1-379)
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北京 101111
Quick Overview for NSUN4 抗体 (AA 1-379) (ABIN7869274)
抗原
See all NSUN4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-379
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原理
- NSUN4 Antibody / NOP2/Sun RNA methyltransferase 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NSUN4 recombinant protein (Position: M1-K379) was used as the immunogen for the NSUN4 antibody.
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亚型
- IgG
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anti-NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4) (AA 1-384) antibody
NSUN4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4) (AA 26-284) antibodyNSUN4 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4) (N-Term) antibodyNSUN4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4) (AA 71-120) antibodyNSUN4 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4) (N-Term) antibodyNSUN4 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4) (AA 1-379) antibodyNSUN4 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NSUN4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NSUN4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NSUN4 (NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4))
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别名
- NSUN4
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背景
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NSUN4 antibody detects NOP2/Sun RNA methyltransferase 4, encoded by the NSUN4 gene. NOP2/Sun RNA methyltransferase 4 is a mitochondrial rRNA methyltransferase that modifies 12S rRNA and plays a critical role in ribosome assembly and mitochondrial translation. NSUN4 antibody provides researchers with a specific reagent for studying RNA modification, mitochondrial function, and disease associated with mitochondrial dysfunction.
NOP2/Sun RNA methyltransferase 4 belongs to the NSUN family of RNA cytosine methyltransferases. Research using NSUN4 antibody has shown that it catalyzes 5-methylcytosine modification of mitochondrial 12S rRNA. This modification is required for proper folding of ribosomal RNA and stability of the small ribosomal subunit. NSUN4 also interacts with MTERF4, forming a complex that regulates assembly of the mitochondrial large ribosomal subunit.
Studies with NSUN4 antibody have revealed that knockout of NSUN4 leads to defective mitochondrial ribosome biogenesis, impaired oxidative phosphorylation, and embryonic lethality in model organisms. These findings emphasize its essential role in mitochondrial translation and energy metabolism. Without NSUN4, mitochondrial protein synthesis collapses, resulting in severe cellular dysfunction.
Dysregulation of NOP2/Sun RNA methyltransferase 4 has been linked to human disease. Research using NSUN4 antibody has shown that mutations cause mitochondrial translation defects, leading to neurodevelopmental delay and cardiomyopathy. In cancer biology, altered NSUN4 expression may contribute to metabolic reprogramming, where cancer cells adapt mitochondrial translation to support growth. These observations highlight the dual importance of NSUN4 in normal physiology and pathology.
NSUN4 antibody is widely used in immunofluorescence, western blotting, and immunohistochemistry. Immunofluorescence demonstrates mitochondrial localization, western blotting quantifies protein levels in mitochondrial fractions, and immunohistochemistry shows expression in tissues with high energy demand such as heart and brain. These applications make NSUN4 antibody indispensable in mitochondrial biology research.
By supplying validated NSUN4 antibody reagents, NSJ Bioreagents supports studies into RNA modification, mitochondrial translation, and disease. Detection of NOP2/Sun RNA methyltransferase 4 provides researchers with insight into how RNA modifying enzymes regulate mitochondrial function and pathology. -
UniProt
- Q96CB9
抗原
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