Syntaxin 5 抗体 (AA 1-327)
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Quick Overview for Syntaxin 5 抗体 (AA 1-327) (ABIN7869197)
抗原
See all Syntaxin 5 (STX5) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-327
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原理
- STX5 Antibody / Syntaxin 5
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human STX5 recombinant protein (Position: M1-Q327) was used as the immunogen for the STX5 antibody.
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亚型
- IgG
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anti-Syntaxin 5 (STX5) (AA 1-100) antibody
STX5 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5A6 unconjugated
anti-Syntaxin 5 (STX5) (AA 1-101) antibodySTX5 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 5A6 unconjugated
anti-Syntaxin 5 (STX5) antibodySTX5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 5 (STX5) (AA 55-355) antibodySTX5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 5 (STX5) (AA 179-209), (Middle Region) antibodySTX5 适用: 人, 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 5 (STX5) (AA 180-209) antibodySTX5 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB17857 unconjugated
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应用备注
- Optimal dilution of the STX5 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the STX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Syntaxin 5 (STX5)
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别名
- STX5
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背景
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STX5 antibody detects Syntaxin-5, a membrane-anchored SNARE protein essential for vesicle trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. Syntaxin-5 mediates membrane fusion events within the early secretory pathway and ensures the proper transport of proteins and lipids through the Golgi complex. The STX5 antibody is widely used in studies of intracellular trafficking, Golgi organization, and protein secretion.
Syntaxin-5 is encoded by the STX5 gene located on human chromosome 11q12.2. The protein exists as two isoforms generated by alternative translation initiation, a long (42 kilodalton) and short (35 kilodalton) form, both containing an N-terminal coiled-coil region and a C-terminal transmembrane domain. These isoforms localize to different Golgi subcompartments and coordinate SNARE complex formation with partners such as Bet1, Sec22b, and GosR2 to drive vesicle docking and fusion.
The STX5 antibody detects a characteristic double band in western blot corresponding to both isoforms. Immunofluorescence typically shows strong perinuclear staining consistent with Golgi localization. Functionally, Syntaxin-5 plays a pivotal role in maintaining Golgi structure and retrograde transport from the Golgi to ER. Disruption of STX5 function leads to ER stress, defective glycosylation, and impaired secretion of transmembrane and secretory proteins.
Syntaxin-5 also participates in autophagy and lipid metabolism by regulating membrane flow between organelles. Mutations in STX5 cause congenital disorders of glycosylation and multisystemic developmental delay. The protein further contributes to viral replication processes, as certain viruses exploit STX5-dependent pathways to reorganize host membranes. NSJ Bioreagents provides a validated STX5 antibody optimized for western blot, immunofluorescence, and immunohistochemistry, supporting research into vesicle trafficking, ER-Golgi transport, and cellular homeostasis. -
UniProt
- Q13190
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途径
- Transition Metal Ion Homeostasis, Regulation of Cell Size, Synaptic Vesicle Exocytosis
抗原
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