TFDP3 抗体 (AA 1-305)
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北京 101111
Quick Overview for TFDP3 抗体 (AA 1-305) (ABIN7869171)
抗原
See all TFDP3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-305
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原理
- TFDP3 Antibody / Transcription factor Dp family member 3
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human TFDP3 recombinant protein (Position: M1-E305) was used as the immunogen for the TFDP3 antibody.
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亚型
- IgG
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anti-Transcription Factor Dp Family, Member 3 (TFDP3) (AA 1-100) antibody
TFDP3 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 1F11 unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (AA 61-196) antibodyTFDP3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (AA 71-120) antibodyTFDP3 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (C-Term) antibodyTFDP3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (AA 215-264) antibodyTFDP3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (AA 82-108), (N-Term) antibodyTFDP3 适用: 人 WB 宿主: 兔 Polyclonal RB30504 unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (Middle Region) antibodyTFDP3 适用: 人, 豚鼠, 兔, 大鼠, Cow, 犬, 马, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor Dp Family, Member 3 (TFDP3) (N-Term) antibodyTFDP3 适用: 人, 豚鼠, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TFDP3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TFDP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- TFDP3 (Transcription Factor Dp Family, Member 3 (TFDP3))
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别名
- TFDP3
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背景
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TFDP3 antibody recognizes Transcription factor Dp family member 3, a unique atypical DP protein that modulates E2F-dependent transcription and cell cycle regulation. Unlike canonical DP proteins (TFDP1 and TFDP2), TFDP3 acts as a transcriptional repressor rather than an activator. It binds to E2F transcription factors but lacks the transactivation domain necessary for promoting gene expression, thereby acting as a competitive inhibitor. The TFDP3 antibody is an essential tool for studying cell proliferation, differentiation, apoptosis, and oncogenic transformation regulated through the E2F/DP pathway.
TFDP3 is encoded by the TFDP3 gene located on human chromosome Xq26.2. The protein consists of a conserved DNA-binding domain and a dimerization region that facilitates complex formation with E2F1. This heterodimerization blocks the transcriptional activation of E2F target genes involved in DNA synthesis and cell cycle progression. As a result, TFDP3 contributes to cell cycle arrest and resistance to apoptosis, especially under stress or DNA damage conditions.
The TFDP3 antibody is used to detect the 45-47 kDa nuclear protein in various cell lines and tissues. Immunofluorescence reveals nuclear localization consistent with its function as a transcriptional regulator. TFDP3 is predominantly expressed in testis, certain stem cells, and a subset of cancer tissues. Its overexpression has been reported in hepatocellular carcinoma, lung adenocarcinoma, and breast cancer, where it promotes survival by suppressing E2F1-induced apoptosis. Mechanistically, TFDP3 interferes with E2F1's ability to induce p53-independent cell death, thereby facilitating oncogenic proliferation.
Beyond cell cycle regulation, TFDP3 participates in differentiation processes and developmental gene expression control. It is also implicated in DNA repair regulation and stress-induced transcriptional reprogramming. Studies have shown that silencing TFDP3 enhances the cytotoxic effects of chemotherapeutic agents, making it a potential therapeutic target. NSJ Bioreagents offers a validated TFDP3 antibody optimized for western blot, immunohistochemistry, and chromatin immunoprecipitation, providing researchers with a robust reagent for dissecting E2F pathway regulation and its role in tumor biology. -
UniProt
- Q5H9I0
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途径
- Cell Division Cycle
抗原
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