POLR1E 抗体 (AA 1-291)
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北京 101111
Quick Overview for POLR1E 抗体 (AA 1-291) (ABIN7869059)
抗原
See all POLR1E 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-291
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原理
- POLR1E Antibody / DNA-directed RNA polymerase I subunit E / PAF53
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) was used as the immunogen for the POLR1E antibody.
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亚型
- IgG
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anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (AA 1-419) antibody
POLR1E 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (Internal Region) antibodyPOLR1E 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (AA 1-419) antibodyPOLR1E 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (AA 35-84) antibodyPOLR1E 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) antibodyPOLR1E 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (AA 171-198) antibodyPOLR1E 适用: 人 WB 宿主: 兔 Polyclonal RB37044 unconjugated
anti-Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (N-Term) antibodyPOLR1E 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the POLR1E antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the POLR1E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))
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别名
- POLR1E
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背景
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POLR1E antibody detects DNA-directed RNA polymerase I subunit E (also known as PAF53), a core component of the RNA polymerase I complex responsible for synthesizing ribosomal RNA (rRNA) precursors. Encoded by the POLR1E gene on chromosome 9q34.11, this protein is essential for rRNA transcription and ribosome biogenesis. POLR1E interacts with other polymerase I subunits, transcription initiation factors, and nucleolar scaffolding proteins to regulate transcription of the 47S rRNA gene cluster. By supporting nucleolar organization and transcriptional efficiency, it plays a fundamental role in cell growth and proliferation.
POLR1E localizes to the nucleolus and associates with the transcription initiation factor SL1 and upstream binding factor (UBF) to form a preinitiation complex at rDNA promoters. Its C-terminal domain mediates binding to RNA polymerase I subunit RPA1 and other core enzyme components, facilitating enzyme assembly and promoter clearance. Loss of POLR1E disrupts nucleolar architecture and reduces rRNA synthesis, leading to impaired ribosome production and cell-cycle arrest. This makes it a critical determinant of nucleolar integrity and growth control.
The POLR1E antibody is widely used in molecular biology, cancer research, and nucleolar studies to assess RNA polymerase I activity and rRNA transcription regulation. Western blot analysis identifies a 53 kilodalton band corresponding to POLR1E, while immunofluorescence reveals punctate nucleolar staining. Because RNA polymerase I transcription drives ribosome production, alterations in POLR1E expression are linked to tumor growth and metabolic adaptation. Increased expression has been observed in rapidly dividing cells, whereas downregulation occurs under cellular stress or growth inhibition.
In addition to its role in transcription, POLR1E contributes to the nucleolar stress response by coordinating signaling between rRNA synthesis and p53 activation. Defects in polymerase I assembly or activity trigger nucleolar stress pathways that stabilize p53, resulting in growth arrest. The POLR1E antibody enables exploration of these mechanisms and supports investigations into ribosomopathies, tumorigenesis, and cellular aging. NSJ Bioreagents provides this antibody validated for its applications, ensuring precise and reliable detection across model systems. -
UniProt
- Q9GZS1
抗原
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