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DNMT3A 抗体 (AA 1-284)

Name: DNMT3A 抗体 (AA 1-284)
SKU: ABIN7869046
Availability: OutOfStock

The 兔多克隆 anti-DNMT3A antibody (ABIN7869046) specifically detects DNMT3A in WB, ELISA, IHC, FACS, IF, IP 和 ICC. The antibody is reactive with 人, 小鼠和大鼠 samples.

产品编号 ABIN7869046

发货至: 中国

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Quick Overview for DNMT3A 抗体 (AA 1-284) (ABIN7869046)

抗原

See all DNMT3A 抗体

DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This DNMT3A antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Discover our top product DNMT3A Primary Antibody

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AA 1-284

原理

DNMT3A Antibody / DNA (cytosine-5)-methyltransferase 3A

纯化方法

Immunogen affinity purified

免疫原

E.coli-derived human DNMT3A recombinant protein (Position: M1-Y284) was used as the immunogen for the DNMT3A antibody.

亚型

IgG
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 500-700) antibody
DNMT3A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody
DNMT3A 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB01852 unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 26-100) antibody
DNMT3A 适用: 人, 小鼠, 大鼠 FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 46-180) antibody
DNMT3A 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4G5F11 unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody
DNMT3A 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) antibody
DNMT3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 705-908), (C-Term) antibody
DNMT3A 适用: 人, 小鼠 WB, IHC, ICC, ChIP 宿主: 小鼠 Monoclonal 64B1446 unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) antibody
DNMT3A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 680-902) antibody
DNMT3A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) antibody
KO Validated DNMT3A 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Optimal dilution of the DNMT3A antibody should be determined by the researcher.

限制

仅限研究用
状态

Lyophilized

溶解方式

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

储存条件

4 °C,-20 °C

储存方法

After reconstitution, the DNMT3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
抗原

DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

别名

DNMT3A

背景

DNMT3A antibody detects DNA (cytosine-5)-methyltransferase 3A, an enzyme responsible for establishing de novo DNA methylation patterns during development and differentiation. The UniProt recommended name is DNA (cytosine-5)-methyltransferase 3A (DNMT3A). This nuclear enzyme catalyzes the transfer of a methyl group to the 5-position of cytosine residues within CpG dinucleotides, a key mechanism of epigenetic gene regulation.

Functionally, DNMT3A antibody identifies an 912-amino-acid methyltransferase that functions together with DNMT3B and DNMT3L to establish DNA methylation marks during embryogenesis. DNMT3A binds chromatin through its PWWP domain and ADD (ATRX-DNMT3-DNMT3L) domain, recognizing histone modifications that guide locus-specific methylation. By controlling methylation of promoters, enhancers, and repetitive elements, DNMT3A ensures stable gene silencing and genome integrity.

The DNMT3A gene is located on chromosome 2p23.3 and is highly expressed in stem cells, germ cells, and hematopoietic progenitors. Expression decreases during differentiation, consistent with its role in developmental programming. DNMT3A activity is essential for imprinting, X-chromosome inactivation, and transposon silencing.

Pathologically, mutations in DNMT3A are among the most common in acute myeloid leukemia (AML), myelodysplastic syndromes, and clonal hematopoiesis. Loss-of-function mutations impair methylation fidelity, causing epigenetic instability and aberrant gene expression. Germline mutations lead to Tatton-Brown-Rahman syndrome, characterized by overgrowth and intellectual disability. Research using DNMT3A antibody supports studies in epigenetics, cancer biology, and developmental regulation.

DNMT3A antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect DNA methyltransferases. NSJ Bioreagents provides DNMT3A antibody reagents optimized for studies in epigenetic modification, chromatin remodeling, and transcriptional silencing.

Structurally, DNA (cytosine-5)-methyltransferase 3A consists of an N-terminal regulatory region containing the PWWP and ADD domains, and a C-terminal catalytic domain with the conserved PCQ motif required for methyl group transfer. DNMT3A forms complexes with DNMT3L to enhance DNA binding and catalytic efficiency. This antibody enables investigation of DNMT3A's function in epigenetic gene silencing and oncogenic transformation.

UniProt

Q9Y6K1