TIGAR 抗体 (AA 1-270)
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北京 101111
Quick Overview for TIGAR 抗体 (AA 1-270) (ABIN7869019)
抗原
See all TIGAR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-270
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原理
- TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human TIGAR recombinant protein (Position: M1-R270) was used as the immunogen for the TIGAR antibody.
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亚型
- IgG
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anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 24-188) antibody
TIGAR 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 161-270) antibodyTIGAR 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 220-270) antibodyTIGAR 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 220-270) antibodyTIGAR 适用: 人 IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 220-270) antibodyTIGAR 适用: 人 IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) antibodyTIGAR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-30), (N-Term) antibodyTIGAR 适用: 小鼠 WB 宿主: 兔 Polyclonal RB39908 unconjugated
anti-TP53 induced glycolysis regulatory phosphatase (TIGAR) (Center) antibodyTIGAR 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TIGAR antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TIGAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
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别名
- TIGAR
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背景
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TIGAR antibody detects TP53-induced glycolysis and apoptosis regulator, a key metabolic enzyme that modulates glycolysis, antioxidant defense, and cell survival. Encoded by the TIGAR gene on chromosome 12p13.3, this protein was identified as a transcriptional target of the tumor suppressor p53. TIGAR functions as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate in the cytoplasm and thereby inhibiting glycolysis while promoting the pentose phosphate pathway. This metabolic shift enhances NADPH production, which supports antioxidant defense and protects cells against reactive oxygen species (ROS).
TIGAR contains a bisphosphatase domain homologous to that of the PFKFB family of enzymes but functions independently to regulate metabolic flux. By lowering intracellular fructose-2,6-bisphosphate, it decreases glycolytic activity and reduces lactate production while increasing glucose utilization for nucleotide synthesis and redox balance. This activity allows TIGAR to protect cells from oxidative stress and apoptosis under p53-mediated stress responses. It also contributes to autophagy regulation by balancing energy production and redox homeostasis.
The TIGAR antibody is used in metabolism, cancer, and redox biology research to investigate metabolic reprogramming and p53-dependent signaling. Western blot analysis typically identifies a 30 kilodalton band corresponding to TIGAR, while immunofluorescence shows cytoplasmic and perinuclear staining in metabolically active cells. Because TIGAR regulates the balance between glycolysis and antioxidant pathways, this antibody is a valuable tool for studying stress responses, metabolic adaptation, and tumor metabolism.
In cancer, TIGAR overexpression can support tumor cell survival by reducing oxidative stress, contributing to chemoresistance and metabolic flexibility. Conversely, TIGAR deficiency enhances ROS accumulation and apoptosis, sensitizing cells to stress. NSJ Bioreagents provides the TIGAR antibody validated for its applications, ensuring consistent detection in studies of metabolism, apoptosis, and cancer biology. -
UniProt
- Q9NQ88
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途径
- Warburg Effect
抗原
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