NUDT8 抗体 (AA 1-236)
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Quick Overview for NUDT8 抗体 (AA 1-236) (ABIN7868890)
抗原
See all NUDT8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-236
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原理
- NUDT8 Antibody / Nudix hydrolase 8
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NUDT8 recombinant protein (Position: M1-L236) was used as the immunogen for the NUDT8 antibody.
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亚型
- IgG
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anti-Nudix Hydrolase 8 (NUDT8) (AA 42-70), (N-Term) antibody
NUDT8 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB27955 unconjugated
anti-Nudix Hydrolase 8 (NUDT8) (AA 49-78), (N-Term) antibodyNUDT8 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Nudix Hydrolase 8 (NUDT8) (AA 1-140) antibodyNUDT8 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NUDT8 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NUDT8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NUDT8 (Nudix Hydrolase 8 (NUDT8))
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别名
- NUDT8
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背景
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NUDT8 antibody detects Nudix hydrolase 8, a mitochondrial enzyme encoded by the NUDT8 gene on chromosome 4p14. NUDT8 belongs to the Nudix hydrolase family of pyrophosphatases, which catalyze the hydrolysis of nucleoside diphosphates and related metabolites to prevent accumulation of potentially toxic intermediates. NUDT8 is localized to the mitochondrial matrix, where it plays a role in maintaining nucleotide balance and mitochondrial integrity. It is expressed in energy-demanding tissues such as heart, skeletal muscle, and brain, reflecting its role in nucleotide metabolism and redox regulation.
Structurally, NUDT8 contains the conserved Nudix (nucleoside diphosphate linked to another moiety, X) motif GX5EX7REUXEEXGU, which defines the catalytic site of the Nudix hydrolase family. The enzyme hydrolyzes substrates such as CoA diphosphate derivatives, ADP-ribose, and other nucleotide intermediates. NUDT8 belongs to the mitochondrial subfamily of Nudix enzymes, which also includes NUDT9 and NUDT15. Co-localization studies confirm its mitochondrial matrix localization, consistent with roles in energy metabolism and oxidative stress defense.
Functionally, NUDT8 acts as a metabolic housekeeper by removing oxidized or excess nucleotide derivatives that can disrupt mitochondrial function. It maintains CoA and ADP-ribose homeostasis, supporting efficient energy production and mitochondrial redox balance. NUDT8 also contributes to nucleotide quality control by hydrolyzing damaged substrates generated during oxidative stress. Through this activity, it supports mitochondrial DNA replication fidelity and prevents accumulation of noncanonical nucleotides.
NUDT8 expression is developmentally regulated, increasing during mitochondrial biogenesis and metabolic activation in muscle and neuronal cells. Dysregulation of NUDT8 has been linked to metabolic disorders and mitochondrial dysfunction. Reduced enzyme activity can impair oxidative phosphorylation, while overexpression enhances resilience against oxidative damage. Pathway involvement includes CoA metabolism, NAD metabolism, and nucleotide salvage pathways.
The NUDT8 antibody from NSJ Bioreagents is a valuable reagent for research into mitochondrial metabolism, nucleotide regulation, and redox homeostasis. -
UniProt
- Q8WV74
抗原
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