HDHD1 抗体 (AA 1-228)
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Quick Overview for HDHD1 抗体 (AA 1-228) (ABIN7868873)
抗原
See all HDHD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-228
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原理
- PUDP Antibody / Pseudouridine 5-phosphatase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PUDP recombinant protein (Position: M1-E228) was used as the immunogen for the PUDP antibody.
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亚型
- IgG
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anti-Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1) antibody
HDHD1 适用: 人, 犬, 猴, 大鼠, 小鼠 WB, FACS, IHC (p), IHC 宿主: 小鼠 Monoclonal 7A2 unconjugated
anti-Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1) (AA 107-212) antibodyHDHD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F12 unconjugated
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应用备注
- Optimal dilution of the PUDP antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PUDP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- HDHD1 (Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1))
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别名
- PUDP
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背景
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PUDP antibody detects Pseudouridine 5'-phosphatase, an enzyme involved in nucleotide metabolism and RNA modification recycling. Encoded by the PUDP gene on chromosome 10q23.31, this enzyme catalyzes the dephosphorylation of pseudouridine 5'-phosphate, a modified nucleoside derived from RNA turnover. By recycling pseudouridine into usable intermediates, PUDP contributes to nucleotide homeostasis and RNA degradation pathways essential for cellular metabolism.
PUDP is a member of the haloacid dehalogenase (HAD) superfamily of hydrolases and shares structural features with sugar phosphatases, including a conserved catalytic Asp residue that facilitates phosphate hydrolysis. The enzyme functions primarily in the cytoplasm and exhibits substrate specificity for pseudouridine monophosphate over canonical nucleotides. PUDP activity supports RNA salvage by clearing modified nucleosides generated from rRNA and tRNA decay.
The PUDP antibody is used in enzymology, RNA metabolism, and nucleotide biochemistry research to study RNA turnover and modified nucleotide processing. Western blot analysis identifies a 31 kilodalton band corresponding to PUDP, while immunofluorescence shows diffuse cytoplasmic localization consistent with its metabolic role. This antibody provides a useful reagent for monitoring nucleotide recycling and evaluating enzymatic regulation in metabolic and stress-response pathways.
Altered expression of PUDP has been associated with cancer, where metabolic reprogramming changes nucleotide flux and RNA stability. By participating in modified nucleotide clearance, PUDP helps maintain nucleotide pool balance and prevents accumulation of potentially toxic intermediates. The PUDP antibody supports studies into RNA degradation, nucleotide recycling, and metabolic adaptation. NSJ Bioreagents validates this antibody for its applications ensuring specificity and reproducibility for nucleotide metabolism research. -
UniProt
- Q08623
抗原
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