GINS4 抗体 (AA 1-223)
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北京 101111
Quick Overview for GINS4 抗体 (AA 1-223) (ABIN7868852)
抗原
See all GINS4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-223
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原理
- GINS4 Antibody / GINS complex subunit 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SLD5/GINS4 recombinant protein (Position: M1-I223) was used as the immunogen for the GINS4 antibody.
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亚型
- IgG
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anti-GINS Complex Subunit 4 (Sld5 Homolog) (GINS4) (AA 2-223) antibody
GINS4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GINS Complex Subunit 4 (Sld5 Homolog) (GINS4) antibodyGINS4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-GINS Complex Subunit 4 (Sld5 Homolog) (GINS4) (AA 108-207) antibodyGINS4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-GINS Complex Subunit 4 (Sld5 Homolog) (GINS4) (AA 173-23) antibodyGINS4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the GINS4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the GINS4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- GINS4 (GINS Complex Subunit 4 (Sld5 Homolog) (GINS4))
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别名
- GINS4
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背景
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GINS4 antibody detects GINS complex subunit 4, encoded by the GINS4 gene. GINS complex subunit 4, also known as SLD5, is one of four essential components of the GINS complex, which plays a central role in DNA replication initiation and elongation. GINS4 antibody provides researchers with a specific reagent for studying DNA replication, genome stability, and cell cycle control.
The GINS complex, composed of GINS1, GINS2, GINS3, and GINS4, forms a stable heterotetramer that interacts with CDC45 and the MCM helicase complex to establish the CMG helicase. Research using GINS4 antibody has demonstrated that the CMG helicase is the core replicative helicase in eukaryotic cells, responsible for unwinding DNA during replication. Loss of GINS4 impairs DNA replication fork progression and genome stability, highlighting its indispensable role.
Studies with GINS4 antibody have revealed that GINS4 interacts with multiple replication factors, including MCM proteins, replication protein A, and DNA polymerases. These interactions coordinate helicase activity with DNA synthesis, ensuring high-fidelity replication. Structural studies further confirm that GINS4 stabilizes the complex and supports helicase activity.
Dysregulation of GINS4 is linked to cancer and genomic instability. Research using GINS4 antibody has shown that elevated GINS4 expression is associated with proliferation and poor prognosis in several cancers, including lung and colorectal cancers. By promoting replication efficiency, cancer cells exploit GINS4 to sustain high growth rates. Conversely, depletion of GINS4 induces replication stress and apoptosis, underscoring its therapeutic potential as a replication factor target.
GINS4 antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting quantifies protein levels, immunohistochemistry identifies proliferative zones with high replication activity, and immunoprecipitation demonstrates interaction with replication machinery. These approaches make GINS4 antibody invaluable in DNA replication research.
By supplying validated GINS4 antibody reagents, NSJ Bioreagents supports studies into DNA replication, cell cycle regulation, and cancer biology. Detection of GINS complex subunit 4 provides insight into how replication machinery safeguards genome stability. -
UniProt
- Q9BRT9
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途径
- DNA Replication, Synthesis of DNA
抗原
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