CD69 抗体 (AA 1-199)
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北京 101111
Quick Overview for CD69 抗体 (AA 1-199) (ABIN7868359)
抗原
See all CD69 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-199
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原理
- CD69 Antibody
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human CD69 recombinant protein (Position: M1-K199) was used as the immunogen for the CD69 antibody.
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亚型
- IgG
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anti-CD69 (CD69) (AA 101-199) antibody
CD69 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD69 (CD69) antibodyCD69 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8B6 unconjugated
anti-CD69 (CD69) (AA 1-199) antibodyCD69 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD69 (CD69) (AA 64-199) antibodyCD69 适用: 人 FACS, WB, ICC 宿主: 兔 Monoclonal BLR170J unconjugated Recombinant Antibody
anti-CD69 (CD69) antibodyCD69 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal FN50 unconjugated
anti-CD69 (CD69) (AA 101-148) antibodyCD69 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD69 (CD69) (Internal Region) antibodyCD69 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD69 (CD69) (AA 90-199) antibodyCD69 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 4H3 unconjugated
anti-CD69 (CD69) antibody (PE)CD69 适用: 人, Baboon, Cynomolgus, Macaque, 猕猴, Chimpanzee FACS 宿主: 小鼠 Monoclonal FN50 PE
anti-CD69 (CD69) (AA 62-199) antibodyCD69 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the CD69 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the CD69 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- CD69
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别名
- CD69
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背景
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CD69 antibody detects Early activation antigen CD69, encoded by the CD69 gene on chromosome 12p13.31. CD69 antibody is widely used in immunology research as CD69 is one of the earliest markers of lymphocyte activation. CD69 is a type II transmembrane C-type lectin receptor expressed on T cells, B cells, NK cells, and other leukocytes shortly after stimulation. It functions in immune regulation, signaling, and tissue retention of lymphocytes, making it a key marker of immune activation and inflammation.
Structurally, CD69 is a ~28-32 kDa disulfide-linked homodimer composed of an extracellular C-type lectin-like domain, a transmembrane region, and a short cytoplasmic tail. The lectin domain mediates protein interactions, while the transmembrane region associates with signaling molecules. CD69 is rapidly upregulated at the cell surface following antigen recognition or cytokine stimulation, making it a sensitive marker of early activation.
Functionally, CD69 regulates immune cell proliferation, cytokine production, and migration. It modulates signaling pathways downstream of the T cell receptor and integrins. Importantly, CD69 binds sphingosine-1-phosphate receptor 1 (S1PR1) and promotes its internalization, reducing lymphocyte egress from lymphoid organs. Through this mechanism, CD69 contributes to tissue retention of immune cells during immune responses. Researchers use CD69 antibody to study lymphocyte activation, tissue homing, and inflammation.
Clinically, CD69 is an important biomarker of immune activation in infection, autoimmune disease, and cancer. Elevated CD69 expression is associated with autoimmune pathologies such as lupus and rheumatoid arthritis. It has also been used to monitor immune responses in HIV infection and cancer immunotherapy. In oncology, tumor-infiltrating lymphocytes expressing CD69 often indicate active immune engagement. NSJ Bioreagents provides CD69 antibody for immunology, oncology, and translational research applications.
Experimentally, CD69 antibody is widely used in flow cytometry to monitor lymphocyte activation, in immunohistochemistry to study tissue infiltration, and in western blotting to confirm expression. Functional assays using CD69 antibody help evaluate lymphocyte responses to antigenic or cytokine stimuli. -
UniProt
- Q07108
抗原
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