Feimin 抗体
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北京 101111
Quick Overview for Feimin 抗体 (ABIN7866182)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-Feimin (MOUSE) Monoclonal Antibody
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交叉反应 （详细）
- A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.
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纯化方法
- Anti-Feimin Monoclonal Antibody is Protein A purified
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过滤
- Sterile filtered
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免疫原
- This monoclonal antibody was prepared in mice after repeated immunizations with recombinant mouse Feimin protein.
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亚型
- IgG2b, kappa
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anti-Feimin (pThr112) antibody
适用: 小鼠 ELISA, SDS 宿主: 小鼠 Monoclonal unconjugated
anti-Feimin antibody适用: 小鼠, 人 ELISA, IP, WB 宿主: 兔 Polyclonal unconjugated
anti-Feimin (N-Term) antibody适用: 人 ELISA, IP, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Anti-Feimin (MOUSE) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T). A band approximately 17kDa for isoform 2 and 20kDa fo
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说明
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ELISA Dilution: 1:265,000-1:409,000
Western Blot Dilution: 41:5000br />Immunoprecipitation Dilution: 40 ng/mL -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.16 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for
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有效期
- 12 months
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- Feimin
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背景
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Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin -
基因ID
- 134553
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NCBI登录号
- NP_001129058.1
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UniProt
- Q80X32
抗原
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