CD2 抗体
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北京 101111
Quick Overview for CD2 抗体 (ABIN7866122)
抗原
See all CD2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse anti Human CD2
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产品特性
- Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.
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纯化方法
- Protein A/G Chromatography
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免疫原
- Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.
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亚型
- IgG2a
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应用备注
- Flow cytometry: PBMC: Add 10 μL of antibody to 1 million PBMCs in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 μL of antibody to 100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for immunofluorescence analysis with a flow cytometer or microscope.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- CD2
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别名
- CD2
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背景
- T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein
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UniProt
- P06729
抗原
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