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CD2 抗体

The 小鼠 单克隆 anti-CD2 antibody is suitable to detect CD2 in samples from 人. It has been validated for FACS.
产品编号 ABIN7866122
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD2 抗体 (ABIN7866122)

抗原

See all CD2 抗体
CD2

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD2 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS)

克隆位点

T6-3
  • 原理

    Mouse anti Human CD2

    产品特性

    Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

    纯化方法

    Protein A/G Chromatography

    免疫原

    Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.

    亚型

    IgG2a
  • 应用备注

    Flow cytometry: PBMC: Add 10 μL of antibody to 1 million PBMCs in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 μL of antibody to 100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for immunofluorescence analysis with a flow cytometer or microscope.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Provided as solution in phosphate buffered saline with 0.08 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
  • 抗原

    CD2

    别名

    CD2

    背景

    T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

    UniProt

    P06729
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