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CD43 抗体

This 小鼠 单克隆 antibody specifically detects CD43 in FACS, WB, IHC, IF 和 ICC. It exhibits reactivity toward 人.
产品编号 ABIN7865735
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD43 抗体 (ABIN7865735)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

84-3C1
  • 原理

    anti-CD43 mouse monoclonal, 84-3C1, purified

    纯化方法

    affinity chromatography

    免疫原

    stimulated human leucocytes

    亚型

    IgG1 kappa
  • 应用备注

    ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

    FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

    IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    WB_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

    IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    CD43

    背景

    84-3C1 reacts with a 95/115 kDa protein on T-cells and thymocytes and a 115/135 kDa molecule on neutrophils and platelets. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. In addition, expression is altered in Wiskott Aldrich syndrome. A proportion of AIDS patients have antibodies to CD43. A soluble form called galactoglycoprotein is present in serum. 85-3C1 was typed at the 3rd International Workshop on Human Leucocyte Differentiation antigens. Positive control: Paracortex in a tonsil or a reactive lymph node.

    途径

    Regulation of Leukocyte Mediated Immunity
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