Retinol-Binding Protein 抗体
The 小鼠 单克隆 anti-Retinol-Binding Protein antibody is suitable to detect Retinol-Binding Protein in samples from 小鼠, 山羊, 猴, 大鼠 和 兔. It has been validated for IHC, WB 和 ELISA.
产品编号 ABIN7865727
发货至:
中国
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中国
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Quick Overview for Retinol-Binding Protein 抗体 (ABIN7865727)
抗原
See all Retinol-Binding Protein (RBP) 抗体
Retinol-Binding Protein (RBP)
适用
小鼠, 山羊, 猴, 大鼠, 兔
宿主
小鼠
克隆类型
单克隆
标记
This Retinol-Binding Protein antibody is un-conjugated
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), ELISA
克隆位点
G4E4
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原理
- anti-Retinol Binding Protein mouse monoclonal, G4E4, purified
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纯化方法
- affinity chromatography
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免疫原
- with human retinol binding protein purified from plasma
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亚型
- IgG1 kappa
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anti-Retinol-Binding Protein (RBP) (AA 45-135) antibody
RBP 适用: 小鼠 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:100-1:200 (0.5-1 μg/mL)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- Retinol-Binding Protein (RBP)
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别名
- Retinol Binding Protein
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背景
- G4E4 recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver. Positive control: Liver.
抗原
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