Proliferation Marker 抗体
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北京 101111
Quick Overview for Proliferation Marker 抗体 (ABIN7865722)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Proliferation Marker mouse monoclonal, IPO-38, purified
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纯化方法
- affinity chromatography
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免疫原
- spleen cells of a patient with hairy cell leukemia
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亚型
- IgM kappa
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anti-Proliferation Marker antibody
适用: 人 IHC, WB, IF 宿主: 小鼠 Monoclonal JC1 unconjugated
anti-Proliferation Marker antibody适用: 人 IHC, WB, IHC (p), ELISA, IP, IHC (fro) 宿主: 小鼠 Monoclonal IPO-38 unconjugated
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应用备注
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IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- Proliferation Marker
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背景
- IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkin’s disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker. Positive control: Raji cells.
抗原
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