Keratin 5/14 抗体
The 小鼠 单克隆 anti-Keratin 5/14 antibody (Clone EBS-IF-001) (ABIN7865680) specifically detects Keratin 5/14 in IHC, ICC, IF, FACS 和 WB.
The antibody is reactive with 人 和 Cat samples.
产品编号 ABIN7865680
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Keratin 5/14 抗体 (ABIN7865680)
抗原
Keratin 5/14 (KRT5/14)
适用
人, Cat
宿主
小鼠
克隆类型
单克隆
标记
This Keratin 5/14 antibody is un-conjugated
应用范围
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)
克隆位点
EBS-IF-001
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原理
- anti-Keratin 5+14 mouse monoclonal, EBS-IF-001, purified
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纯化方法
- affinity chromatography
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免疫原
- cytokeratin-enriched extract of feline tongue epithelium
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亚型
- IgG2a kappa
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应用备注
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
WB_dilution: assay dependent
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- Keratin 5/14 (KRT5/14)
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别名
- Keratin 5+14
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背景
- EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells. Positive control: Tongue.
抗原
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