KRT4 抗体
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北京 101111
Quick Overview for KRT4 抗体 (ABIN7865679)
抗原
See all KRT4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Keratin 4,5,6,8,10,13,18 mouse monoclonal, C-11, purified
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纯化方法
- affinity chromatography
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免疫原
- cytoskeleton preparation of human A431 carcinoma cell line
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/mL PBS recommended)
WB_dilution: 1:100 (1 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- KRT4 (Keratin 4 (KRT4))
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别名
- Keratin 4
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背景
- C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Positive control: A431 cells, skin, lung.
抗原
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