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KRT4 抗体

The 小鼠 单克隆 anti-KRT4 antibody is suitable to detect KRT4 in samples from 人, 大鼠, 小鼠, 犬, Cat, 兔, 山羊, 豚鼠, 猴, 小鸡, 雪貂 和 非洲爪蟾. It has been validated for WB, IHC, IF, FACS 和 ICC.
产品编号 ABIN7865679
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for KRT4 抗体 (ABIN7865679)

抗原

See all KRT4 抗体
KRT4 (Keratin 4 (KRT4))

适用

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  • 5
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  • 1
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人, 大鼠, 小鼠, 犬, Cat, 兔, 山羊, 豚鼠, 猴, 小鸡, 雪貂, 非洲爪蟾

宿主

  • 90
  • 31
  • 1
小鼠

克隆类型

  • 65
  • 57
单克隆

标记

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This KRT4 antibody is un-conjugated

应用范围

  • 81
  • 61
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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

C-11
  • 原理

    anti-Keratin 4,5,6,8,10,13,18 mouse monoclonal, C-11, purified

    纯化方法

    affinity chromatography

    免疫原

    cytoskeleton preparation of human A431 carcinoma cell line

    亚型

    IgG1 kappa
  • 应用备注

    ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

    FACS_dilution: 0.5-1.0 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

    IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/mL PBS recommended)

    WB_dilution: 1:100 (1 μg/mL)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    KRT4 (Keratin 4 (KRT4))

    别名

    Keratin 4

    背景

    C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Positive control: A431 cells, skin, lung.
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