Keratin 10/13 抗体
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北京 101111
Quick Overview for Keratin 10/13 抗体 (ABIN7865677)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Keratin-13+10 mouse monoclonal, EBS-IF-003, purified
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纯化方法
- affinity chromatography
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免疫原
- cytoskeleton preparation from HeLa cells
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亚型
- IgG2a kappa
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anti-Keratin 10/13 antibody
适用: 人, Cat, 斑马鱼 WB, FACS, IHC, IHC (p), IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IHC, IHC (p), IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
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应用备注
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonon vor 15 min, at 4 °C
IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- Keratin 10/13
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别名
- Keratin-13+10
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背景
- EBS-IF-003 reacts with 53 kDa (CK13) and 56.6 kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis. Positive control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
抗原
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