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ASRGL1 抗体

This 小鼠 单克隆 antibody specifically detects ASRGL1 in IHC, WB 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN7865675
发货至: 中国
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Quick Overview for ASRGL1 抗体 (ABIN7865675)

抗原

ASRGL1 (Isoaspartyl Peptidase/L-Asparaginase (ASRGL1))

适用

宿主

  • 1
小鼠

克隆类型

  • 1
单克隆

标记

  • 1
This ASRGL1 antibody is un-conjugated

应用范围

  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), ELISA

克隆位点

7B6
  • 原理

    anti-CRASH mouse monoclonal, 7B6, purified

    纯化方法

    affinity chromatography

    免疫原

    with CRASH recombinant protein and synthetic peptide

    亚型

    IgG1 kappa
  • 应用备注

    IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    WB_dilution: 1:50-1:100 (1-2 μg/mL)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    elisa_dilution: assay dependent

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    ASRGL1 (Isoaspartyl Peptidase/L-Asparaginase (ASRGL1))

    别名

    CRASH

    背景

    7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32 % homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa. Positive control: Ovarian cancer, KM22 breast cancer cells.
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