ASRGL1 抗体
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北京 101111
Quick Overview for ASRGL1 抗体 (ABIN7865675)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CRASH mouse monoclonal, 7B6, purified
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纯化方法
- affinity chromatography
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免疫原
- with CRASH recombinant protein and synthetic peptide
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亚型
- IgG1 kappa
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应用备注
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IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- ASRGL1 (Isoaspartyl Peptidase/L-Asparaginase (ASRGL1))
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别名
- CRASH
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背景
- 7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32 % homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa. Positive control: Ovarian cancer, KM22 breast cancer cells.
抗原
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