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ICAM-3/CD50 抗体

This 小鼠 单克隆 antibody specifically detects ICAM-3/CD50 in FACS, IHC, IF 和 ICC. It exhibits reactivity toward 人.
产品编号 ABIN7865666
发货至: 中国
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Room 801-803
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Quick Overview for ICAM-3/CD50 抗体 (ABIN7865666)

抗原

See all ICAM-3/CD50 (ICAM3) 抗体
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

适用

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宿主

  • 113
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小鼠

克隆类型

  • 138
  • 34
单克隆

标记

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This ICAM-3/CD50 antibody is un-conjugated

应用范围

  • 94
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  • 50
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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

189-2G9
  • 原理

    anti-CD50 mouse monoclonal, 189-2G9, purified

    纯化方法

    affinity chromatography

    免疫原

    stimulated human leucocytes

    亚型

    IgG2b kappa
  • 应用备注

    ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

    FACS_dilution: 1-2 μg/million cells

    IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    别名

    CD50

    背景

    189-2G9 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 120 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PI-anchored. 186-2G9 has been clustered at the VIth GLDA International Workshop. 186-2D9 recognized the D1 domain of human CD50 and blocks binding of CD11a (α L-integrin) to CD50. Positive control: HL-60 or THP-1 cells. Lymph node and tonsil.
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