ICAM-3/CD50 抗体
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北京 101111
Quick Overview for ICAM-3/CD50 抗体 (ABIN7865666)
抗原
See all ICAM-3/CD50 (ICAM3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CD50 mouse monoclonal, 189-2G9, purified
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纯化方法
- affinity chromatography
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免疫原
- stimulated human leucocytes
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亚型
- IgG2b kappa
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应用备注
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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别名
- CD50
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背景
- 189-2G9 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 120 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PI-anchored. 186-2G9 has been clustered at the VIth GLDA International Workshop. 186-2D9 recognized the D1 domain of human CD50 and blocks binding of CD11a (α L-integrin) to CD50. Positive control: HL-60 or THP-1 cells. Lymph node and tonsil.
抗原
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