CD45RB 抗体
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北京 101111
Quick Overview for CD45RB 抗体 (ABIN7865594)
抗原
See all CD45RB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CD45RB mouse monoclonal, Bra11 (Bra11G), purified
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交叉反应 (详细)
- negative with rat, rhesus monkey
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纯化方法
- affinity chromatography
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免疫原
- non-T, non-B CALLA positive ALL cell line REH
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL)
WB_dilution: assay dependent
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- CD45RB
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别名
- CD45RB
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背景
- CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). Bra11 reacts with CD45RB, which is expressed on mature B-lymphocytes and the majority of lymphomas and leukemia's of B-cell origin. CD45RB is also expressed on cells of myeloid lineage. Positive control: Ramos, U-698, or GA-10 cells. Tonsil.
抗原
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