CD45RA 抗体
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北京 101111
Quick Overview for CD45RA 抗体 (ABIN7865593)
抗原
See all CD45RA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CD45RA mouse monoclonal, 158-4D3, purified
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纯化方法
- affinity chromatography
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免疫原
- stimulated human leucocytes
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亚型
- IgG2a kappa
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anti-CD45RA (CD45RA) antibody
CD45RA 适用: 人 FACS, WB, IHC (p), IP, MS 宿主: 小鼠 Monoclonal MEM-56 unconjugated
anti-CD45RA (CD45RA) antibody (PE)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 PE
anti-CD45RA (CD45RA) antibody (FITC)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 FITC
anti-CD45RA (CD45RA) antibody (APC)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 APC
anti-CD45RA (CD45RA) antibodyCD45RA 适用: 人 IHC 宿主: 小鼠 Monoclonal 472M unconjugated
anti-CD45RA (CD45RA) antibodyCD45RA 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal B-C15 unconjugated
anti-CD45RA (CD45RA) antibody (FITC)CD45RA 适用: 人 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal FITC
anti-CD45RA (CD45RA) antibody (Biotin)CD45RA 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-56 Biotin
anti-CD45RA (CD45RA) antibody (PerCP)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 PerCP
anti-CD45RA (CD45RA) antibodyGMP Grade CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal OTH-74D4 unconjugated
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- CD45RA
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别名
- CD45RA
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背景
- CD45 glycoprotein have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cell multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45 RB (exon 5, CD45RC (exon 6) and CD45R or CD45RO (exons 4-6 spliced out). 158-4D3 was clustered in Boston at the Vth International CD workshop as anti CD45RA, useful to differentiate "naive" T-cells from activated (CD45R0+ve) T-cells, or to differentiate T-cell lymphomas (CD45R0+ve) from B-cell lymphomas (CD45RA+ve). Positive control: Daudi, Raji, IM9 and human lymphocytes. Human lymph node and tonsil.
抗原
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