alpha Fetoprotein 抗体
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Quick Overview for alpha Fetoprotein 抗体 (ABIN7865556)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Alpha fetoprotein (AFP) mouse monoclonal, C3, purified
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交叉反应 (详细)
- not with cow, mouse, rat
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纯化方法
- affinity chromatography
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免疫原
- alpha fetoprotein isolated from serum of hepatoma patients
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亚型
- IgG2a kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- Alpha fetoprotein (AFP)
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背景
- C3 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nunstad to a group of antibodies with low affinity to human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa and expressed in fetal liver but normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some other germ cell tumors and in hepatocellular carcinomas. Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.
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途径
- C21-Steroid Hormone Metabolic Process
抗原
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