ACTH 抗体 (AA 1-24)
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Quick Overview for ACTH 抗体 (AA 1-24) (ABIN7865553)
抗原
See all ACTH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-24
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原理
- anti-ACTH mouse monoclonal, 2F6, purified
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纯化方法
- affinity chromatography
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免疫原
- ACTH synthetic peptide (aa 1-24) conjugated to KLH
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- ACTH (Adrenocorticotropic hormone (ACTH))
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别名
- ACTH
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背景
- ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH), does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Positive control: Pituitary gland, pituitary tumor.
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
抗原
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