DNA-RNA Hybrid 抗体
The 小鼠 单克隆 anti-DNA-RNA Hybrid antibody is suitable to detect DNA-RNA Hybrid in samples from Various Species 和 人. It has been validated for ChIP, ICC, IF, FACS 和 IP.
产品编号 ABIN7865241
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for DNA-RNA Hybrid 抗体 (ABIN7865241)
抗原
DNA-RNA Hybrid
适用
Various Species, 人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)
克隆位点
S9-6
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原理
- DNA-RNA Hybrid Antibody
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特异性
- We have not tested this antibody in-house in Immunofluorescence, CHIP, Immunocytochemistry, Immunoprecipitation or Flow Cytometry. All application recommendations come from publications using this clone. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate.
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免疫原
- DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase
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亚型
- IgG2a, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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- DNA-RNA Hybrid
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背景
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DNA-RNA Duplex, DNA/RNA Duplex, DNA:RNA Duplex, DNA-RNA Hybrid, DNA/RNA Hybrid, DNA:RNA Hybrid, RNA-DNA Duplex, RNA/DNA Duplex, RNA:DNA Duplex, RNA-DNA Hybrid, RNA/DNA Hybrid, RNA:DNA Hybrid
All. Target DNA-RNA heteroduplex (R loop) structure is not sequence-specific or species-specific.
抗原
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