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Recombinant pan Keratin 抗体

The 兔 单克隆 anti-pan Keratin antibody is suitable to detect pan Keratin in samples from 人. It has been validated for IHC 和 WB.
产品编号 ABIN7863742
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant pan Keratin 抗体 (ABIN7863742)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

抗体类型

Recombinant Antibody

适用

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克隆类型

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单克隆

标记

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This pan Keratin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB)

克隆位点

Cocktail PCK-4933R
  • 原理

    Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

    特异性

    PCK/4933R demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/4933R is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/4933R is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Crude cytokeratin extract prepared from RT-4 and MCF-7 cells

    亚型

    IgG, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    pan Keratin (panKRT)

    别名

    Cytokeratin, pan

    背景

    KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

    adeno- or squamous carcinomas (IHC)., Skin

    分子量

    40-67kDa ()
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