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Recombinant pan Keratin 抗体

This 小鼠 单克隆 antibody specifically detects pan Keratin in IF, IHC, FACS 和 WB. It exhibits reactivity toward 人, 犬 和 Cat.
产品编号 ABIN7863730
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant pan Keratin 抗体 (ABIN7863730)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

抗体类型

Recombinant Antibody

适用

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人, 犬, Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This pan Keratin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

克隆位点

Cocktail rPCK-6750
  • 原理

    Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

    特异性

    Anti-cytokeratin clone rPCK/6750 (CK rPCK/6750) demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

    亚型

    IgG1, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    pan Keratin (panKRT)

    别名

    Cytokeratin, pan

    背景

    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

    adeno- or squamous carcinomas (IHC)., Skin, A431.

    分子量

    40-67kDa ()
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