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pan Keratin 抗体

The 小鼠 单克隆 anti-pan Keratin antibody (Clone AE-1-AE-3) (ABIN7863711) specifically detects pan Keratin in IF, IHC, FACS 和 WB. The antibody is reactive with 人 samples.
产品编号 ABIN7863711
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for pan Keratin 抗体 (ABIN7863711)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This pan Keratin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

克隆位点

AE-1-AE-3
  • 原理

    Cytokeratin, pan (Epithelial Marker) Antibody

    特异性

    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

    纯化方法

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Human epidermal keratin

    亚型

    IgG1, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    pan Keratin (panKRT)

    别名

    Cytokeratin, pan

    背景

    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

    Skin

    分子量

    40-67kDa

    基因ID

    374454, 51350, 334989

    UniProt

    Q7Z794, Q01546
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