MBD3 抗体
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北京 101111
Quick Overview for MBD3 抗体 (ABIN7863246)
抗原
See all MBD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MBD3 Antibody
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特异性
- Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5' cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, they include methyl-CpG binding protein 1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. MBD1 binds in a context specific manner to methyl-CpG rich domains and, in turn, mediates the transcriptional inhibition that is commonly observed with DNA methylation. Similarly, MBD2 inhibits transcription of methylated genes by associating with histone deacetylase (HDAC1) within the MeCP1 repressor complex. In addition, MBD4, which is also designated MED1, associates with the mismatch repair protein MLH1 and preferentially binds to methylated cytosine residues in mismatched base pairs. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant full-length human MBD3 protein
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亚型
- IgG2b
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anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 79-209) antibody
MBD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB695 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB10 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 70-179) antibodyMBD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-291) antibodyMBD3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibodyMBD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 265-291) antibodyMBD3 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-255) antibodyMBD3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 264-291), (C-Term) antibodyMBD3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02375 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
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别名
- MBD3
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背景
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Methyl CpG binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3
HeLa or K562 cells.
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分子量
- 34kDa
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基因ID
- 53615, 178728
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UniProt
- O95983
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途径
- Chromatin Binding
抗原
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