Recombinant Cyclin B1 抗体
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Quick Overview for Recombinant Cyclin B1 抗体 (ABIN7861160)
抗原
See all Cyclin B1 (CCNB1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Cyclin B1 (G2- & M-phase Cyclin) Antibody
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特异性
- In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephos phorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physio logical substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- His-tagged recombinant hamster CCNB1 protein
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亚型
- IgG, kappa
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anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal CCNB1-1098 unconjugated
anti-Cyclin B1 (CCNB1) (C-Term) antibodyCCNB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 271-433) antibodyCCNB1 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-433) antibodyCCNB1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyKD Validated CCNB1 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 23GB1670 unconjugated Recombinant Antibody
anti-Cyclin B1 (CCNB1) (AA 1-383) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-433) antibodyCCNB1 适用: 人 WB, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-90) antibodyCCNB1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1C8 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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- Cyclin B1 (CCNB1)
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别名
- Cyclin B1
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背景
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CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1
Cell line in logarithmic growth phase. Human tonsil|Prostate or Ovarian Carcinoma.
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分子量
- 55-62kDa
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基因ID
- 891, 23960
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UniProt
- P14635
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途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
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