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Recombinant Choline Acetyltransferase 抗体 (C-Term)

The 小鼠 单克隆 anti-Choline Acetyltransferase antibody (Clone r38B12) (ABIN7857682) specifically detects Choline Acetyltransferase in IHC. The antibody is reactive with 人 samples.
产品编号 ABIN7857682
发货至: 中国
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Quick Overview for Recombinant Choline Acetyltransferase 抗体 (C-Term) (ABIN7857682)

抗原

See all Choline Acetyltransferase (CHAT) 抗体
Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Choline Acetyltransferase antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

R38B12
  • 抗原表位

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    C-Term

    Expression System

    CHO Cells

    原理

    Recombinant Choline Acetyltransferase (CHAT) (Marker of Cholinergic Cells) Antibody

    特异性

    Choline acetyltransferase (ChAT) is a 68 kD enzyme which catalyses the synthesis of acetylcholine (Ach) from choline and acetyl coenzyme A. The human ChAT gene encodes two proteins, the 68 kD ChAT enzyme and a 27 kD protein immunologically related and coexpressed with ChAT in cholinergic neurons of the central nervous system. The smaller proteins may play a role in the regulation of ACh synthesis. ChAT is expressed in cholinergic neurons, the majority of the neurons in the nucleus basalis of Meynert, large neurones in the striatum (putamen and caudate nuclei), the majority of neurones in the pedunculo-pontine, hypoglossal, dorsal nucleus of vagus and subgroups of neurones in Roller,Ä111s and the medial olivary accessory nuclei. Prominent staining is observed in ribonucleoprotein, distributed at the periphery of large neurons of the nucleus basalis of Meynert, the motor neurons in the hypoglossal and ambiguus nuclei. NCL-ChAT does not label axons in the insular cortex of the internal capsule non-cholinergic structures, endothelial cells or microglia.

    纯化方法

    200ug/ml of Ab produced in CHO cell mammalian-based expression system.

    免疫原

    Prokaryotic recombinant protein corresponding to the C-terminal region of the human ChAT molecule.

    亚型

    IgG1, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10  mM PBS

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
  • 抗原

    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

    别名

    Choline Acetyltransferase

    背景

    CMS1A | CMS1A2 CMS6

    Human appendix, colon, stomach or brain.

    分子量

    82kDa

    基因ID

    1103, 410969

    UniProt

    P28329

    途径

    Skeletal Muscle Fiber Development
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