Recombinant Choline Acetyltransferase 抗体 (C-Term)
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Quick Overview for Recombinant Choline Acetyltransferase 抗体 (C-Term) (ABIN7857682)
抗原
See all Choline Acetyltransferase (CHAT) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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Expression System
- CHO Cells
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原理
- Recombinant Choline Acetyltransferase (CHAT) (Marker of Cholinergic Cells) Antibody
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特异性
- Choline acetyltransferase (ChAT) is a 68 kD enzyme which catalyses the synthesis of acetylcholine (Ach) from choline and acetyl coenzyme A. The human ChAT gene encodes two proteins, the 68 kD ChAT enzyme and a 27 kD protein immunologically related and coexpressed with ChAT in cholinergic neurons of the central nervous system. The smaller proteins may play a role in the regulation of ACh synthesis. ChAT is expressed in cholinergic neurons, the majority of the neurons in the nucleus basalis of Meynert, large neurones in the striatum (putamen and caudate nuclei), the majority of neurones in the pedunculo-pontine, hypoglossal, dorsal nucleus of vagus and subgroups of neurones in Roller,Ä111s and the medial olivary accessory nuclei. Prominent staining is observed in ribonucleoprotein, distributed at the periphery of large neurons of the nucleus basalis of Meynert, the motor neurons in the hypoglossal and ambiguus nuclei. NCL-ChAT does not label axons in the insular cortex of the internal capsule non-cholinergic structures, endothelial cells or microglia.
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纯化方法
- 200ug/ml of Ab produced in CHO cell mammalian-based expression system.
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免疫原
- Prokaryotic recombinant protein corresponding to the C-terminal region of the human ChAT molecule.
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亚型
- IgG1, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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别名
- Choline Acetyltransferase
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背景
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CMS1A | CMS1A2 CMS6
Human appendix, colon, stomach or brain.
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分子量
- 82kDa
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基因ID
- 1103, 410969
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UniProt
- P28329
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途径
- Skeletal Muscle Fiber Development
抗原
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