HIST1H2AA 抗体 (PE)
The 大鼠 单克隆 anti-HIST1H2AA antibody is suitable to detect HIST1H2AA in samples from 小鼠. It has been validated for IF 和 FACS.
产品编号 ABIN7841260
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for HIST1H2AA 抗体 (PE) (ABIN7841260)
抗原
See all HIST1H2AA 抗体
HIST1H2AA
(Histone Cluster 1, H2aa (HIST1H2AA))
适用
小鼠
宿主
大鼠
克隆类型
单克隆
标记
This HIST1H2AA antibody is conjugated to PE
应用范围
Immunofluorescence (IF), Flow Cytometry (FACS)
克隆位点
NIMR-4
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原理
- Rat monoclonal antibody PE labled to MHC Class II
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特异性
- Recognizes mouse MHC Class II
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纯化方法
- The antibody was purified by affinity chromatography.
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anti-Histone Cluster 1, H2aa (HIST1H2AA) (AA 2-131) antibody
HIST1H2AA 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow: 10 μL / assay
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Shipped and store at 4°C for one year. Do not freeze.
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- HIST1H2AA (Histone Cluster 1, H2aa (HIST1H2AA))
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别名
- H2-Aa
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背景
- Ia1, H2Aa, Ia-1, H-2Aa, Aalpha, IAalpha, I-Aalpha
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基因ID
- 14960
抗原
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